Goat AP3D1 / Adaptin Delta Polyclonal Antibody | anti-AP3D1 antibody

Goat Polyclonal to Human AP3D1 / Adaptin Delta

Cat. Num. AAA240599

• Gene Names: AP3D1; ADTD; hBLVR
• Reactivity: Chimpanzee, Chicken, Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: AP3D1 / Adaptin Delta; Polyclonal Antibody; Goat Polyclonal to Human AP3D1 / Adaptin Delta; Anti-AP3D1 / Adaptin Delta Antibody (aa686-698) IHC-plus; AP3D1; ADTD; Adaptin; delta; AP-3 complex subunit delta; AP-3 complex subunit delta-1; Delta adaptin; Delta-adaptin; partial CDS; Ap-3 complex delta subunit; HBLVR; Adaptin Delta; Human AP3D1; anti-AP3D1 antibody

• Host: Goat
• Reactivity: Chimpanzee, Chicken, Human
• Clonality: Polyclonal
• Specificity: aa 686-698 of Human AP3D1.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 1153

• Applicable Applications for anti-AP3D1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:5000 - 1:20000), IHC-P (2.5 ug/ml), WB (1:500 - 1:2000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human AP3D1 / AP3.
• Immunogen Type: Synthetic peptide
• Immunogen: AP3D1 / Adaptin Delta antibody was raised against synthetic peptide from human AP3D1 / AP3.
• Antigen Modification: aa686-698
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-AP3D1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-AP3D1 Antibody - Western Blot. Western blot of Affinity Purified anti-AP3D1 antibody shows detection of a 130-kD band corresponding to Human AP3D1 in a HeLa whole cell lysate. The lower molecular weight band most likely represents non-specific binding. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-AP3D1 antibody. Detection occurred using a 1:5000 dilution of HRP-labeled Rabbit anti-Goat IgG for 1 hour at room temperature. A chemilumi-nescence system was used for signal detection (Roche) using a 30-sec exposure time.)

NCBI and Uniprot Product Information

• NCBI GI #: 117553580
• NCBI GeneID: 8943
• NCBI Accession #: NP_003929.4
• NCBI GenBank Nucleotide #: NM_003938.6
• UniProt Accession #: O14617; O00202; O75262; Q59HF5; Q96G11; Q9H3C6
• Molecular Weight: 136,651 Da
• NCBI Official Full Name: AP-3 complex subunit delta-1 isoform 2
• NCBI Official Synonym Full Names: adaptor-related protein complex 3, delta 1 subunit
• NCBI Official Symbol: AP3D1
• NCBI Official Synonym Symbols: ADTD; hBLVR
• NCBI Protein Information: AP-3 complex subunit delta-1; delta adaptin; adaptin, delta; delta-adaptin, partial CDS; AP-3 complex delta subunit, partial CDS; adapter-related protein complex 3 subunit delta-1; adaptor-related protein complex 3 subunit delta-1; subunit of putative vesicle coat adaptor complex AP-3
• UniProt Protein Name: AP-3 complex subunit delta-1
• Protein Family: AP-3 complex
• UniProt Gene Name: AP3D1
• UniProt Entry Name: AP3D1_HUMAN

NCBI Description

The protein encoded by this gene is a subunit of the AP3 adaptor-like complex, which is not clathrin-associated, but is associated with the golgi region, as well as more peripheral structures. The AP-3 complex facilitates the budding of vesicles from the golgi membrane, and may be directly involved in trafficking to lysosomes. This subunit is implicated in intracellular biogenesis and trafficking of pigment granules, and possibly platelet dense granules and neurotransmitter vesicles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

AP3D1: Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Belongs to the adaptor complexes large subunit family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: nucleoplasm; Golgi membrane; Golgi apparatus; membrane coat; membrane; lysosomal membrane; terminal button; endosome membrane

Molecular Function: transporter activity

Biological Process: intracellular protein transport; melanosome organization and biogenesis; protein localization in organelle; anterograde synaptic vesicle transport; antigen processing and presentation, exogenous lipid antigen via MHC class Ib; synaptic vesicle membrane organization and biogenesis; positive regulation of NK T cell differentiation; anterograde axon cargo transport; eye pigment biosynthetic process