Rabbit anti-Human LRP2 (Megalin) Polyclonal Antibody | anti-LRP2 antibody

LRP2 (Megalin) Antibody (C-term)

Cat. Num. AAA9210132

• Gene Names: LRP2; DBS; GP330
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: LRP2 (Megalin); Polyclonal Antibody; LRP2 (Megalin) Antibody (C-term); Low-density lipoprotein receptor-related protein 2; LRP-2; Glycoprotein 330; gp330; Megalin; LRP2; anti-LRP2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This LRP2 (Megalin) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4626-4655 amino acids from the C-terminal region of human LRP2 (Megalin).
• Purity/Purification: Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Concentration: Vial Concentration: 2 (varies by lot)
• Sequence Positions: 4626-4655
• Sequence Length: 4655

• Applicable Applications for anti-LRP2 antibody: ELISA (EIA), Immunohistochemistry (IHC)
• Application Notes: IHC~~1:50~100
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Related Product Information for anti-LRP2 antibody

Low density lipoprotein (LDL) receptor-related protein (LRP), a member of the LDL receptor family, binds multiple classes of ligands and has been implicated in a broad range of normal and disease processes involving lipid metabolism, protease clearance, and cell migration (1). Structurally, members of the LDLR family share homology within their extracellular domains, which are highlighted by the presence of clusters of ligand-binding repeats. LRP is a large endocytic receptor that participates in several biological pathways and plays prominent roles in lipoprotein metabolism and in the catabolism of proteinases involved in coagulation and fibrinolysis. LRP also mediates the cellular entry of certain viruses and toxins and facilitates the activation of various lysosomal enzymes (2). All LRPs are expressed in the central nervous system and, for most receptors, animal models have shown that they are indispensable for successful neurodevelopment. The mechanisms by which they regulate the formation of the nervous system are varied and include the transduction of extracellular signals and the modulation of intracellular signal propagation, as well as cargo transport, the function most commonly attributed to this gene family (3).

Product Categories/Family for anti-LRP2 antibody

Cancer; Cardiovascular; Developmental Biology; Metabolism; Signal Transduction

References

Christensen, E.I., et al., Nat. Rev. Mol. Cell Biol. 3(4):256-266 (2002).
Hjalm, G., et al., Eur. J. Biochem. 239(1):132-137 (1996).
Farquhar, M.G., et al., J. Am. Soc. Nephrol. 6(1):35-47 (1995).
Kounnas, M.Z., et al., J. Biol. Chem. 270(22):13070-13075 (1995).
Korenberg, J.R., et al., Genomics 22(1):88-93 (1994).

NCBI and Uniprot Product Information

• NCBI GI #: 126012573
• NCBI GeneID: 4036
• NCBI Accession #: NP_004516.2
• NCBI GenBank Nucleotide #: NM_004525.2
• UniProt Accession #: P98164; O00711; Q16215
• Molecular Weight: 521958
• NCBI Official Full Name: low-density lipoprotein receptor-related protein 2
• NCBI Official Synonym Full Names: low density lipoprotein receptor-related protein 2
• NCBI Official Symbol: LRP2
• NCBI Official Synonym Symbols: DBS; GP330
• NCBI Protein Information: low-density lipoprotein receptor-related protein 2
• UniProt Protein Name: Low-density lipoprotein receptor-related protein 2
• Protein Family: Low-density lipoprotein receptor-related protein
• UniProt Gene Name: LRP2
• UniProt Synonym Gene Names: LRP-2; gp330
• UniProt Entry Name: LRP2_HUMAN

NCBI Description

The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).[provided by RefSeq, Aug 2009]

Uniprot Description

LRP2: Acts together with cubilin to mediate HDL endocytosis. May participate in regulation of parathyroid- hormone and para-thyroid-hormone-related protein release. Defects in LRP2 are the cause of Donnai-Barrow syndrome (DBS); also known as faciooculoacousticorenal syndrome (FOAR syndrome). DBS is a rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g. agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity. Belongs to the LDLR family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: Golgi apparatus; lysosome; lysosomal membrane; endocytic vesicle; brush border membrane; endoplasmic reticulum; apical plasma membrane; plasma membrane; integral to membrane; coated pit; endosome; receptor complex

Molecular Function: protein binding; calcium ion binding; SH3 domain binding

Biological Process: steroid metabolic process; cell proliferation; phototransduction, visible light; receptor-mediated endocytosis; forebrain development; protein amino acid glycosylation; endocytosis; lipid metabolic process; retinoid metabolic process; vitamin D metabolic process

Disease: Donnai-barrow Syndrome