Paraffin-Embedded
(Figure 1: Staining (paraffin-embedded tissue-sections fixed in formalin) of Megalin in the brush border of the proximal tubule (MBS690201))
Mouse anti-Human Megalin Monoclonal Antibody | anti-LRP2 antibody
Mouse Anti-Human Megalin
The reconstituted antibody is stable for at least two weeks at 2-8°C.
Frozen aliquots are stable for at least 6 months when stored at -20°C.
AVOID REPEATED FREEZE AND THAW CYCLES!
Paraffin-Embedded
(Figure 1: Staining (paraffin-embedded tissue-sections fixed in formalin) of Megalin in the brush border of the proximal tubule (MBS690201))
Paraffin-Embedded
(Figure 1: Staining (paraffin-embedded tissue-sections fixed in formalin) of Megalin in the brush border of the proximal tubule (MBS690201))
Immunofluorescence
(Figure 2: Immunofluorescence staining of Megalin in the brush border of the proximal tubule (MBS690201))
Immunofluorescence
(Figure 2: Immunofluorescence staining of Megalin in the brush border of the proximal tubule (MBS690201))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene, low density lipoprotein-related protein 2 (LRP2) or megalin, is a multi-ligand endocytic receptor that is expressed in many different tissues but primarily in absorptive epithilial tissues such as the kidney. This glycoprotein has a large amino-terminal extracellular domain, a single transmembrane domain, and a short carboxy-terminal cytoplasmic tail. The extracellular ligand-binding-domains bind diverse macromolecules including albumin, apolipoproteins B and E, and lipoprotein lipase. The LRP2 protein is critical for the reuptake of numerous ligands, including lipoproteins, sterols, vitamin-binding proteins, and hormones. This protein also has a role in cell-signaling; extracellular ligands include parathyroid horomones and the morphogen sonic hedgehog while cytosolic ligands include MAP kinase scaffold proteins and JNK interacting proteins. Recycling of this membrane receptor is regulated by phosphorylation of its cytoplasmic domain. Mutations in this gene cause Donnai-Barrow syndrome (DBS) and facio-oculoacoustico-renal syndrome (FOAR).[provided by RefSeq, Aug 2009]
Uniprot Description
Function: Acts together with cubilin to mediate HDL endocytosis
By similarity. May participate in regulation of parathyroid-hormone and para-thyroid-hormone-related protein release.
Subunit structure: Binds plasminogen, extracellular matrix components, plasminogen activator-plasminogen activator inhibitor type I complex, apolipoprotein E-enriched beta-VLDL, lipoprotein lipase, lactoferrin, CLU/clusterin and calcium. Forms a multimeric complex together with a receptor-associated protein (RAP). Binds to ankyrin-repeat family A protein 2 (ANKRA2). Interacts with LRP2BP. Interacts (via NPXY motif) with DAB2; the interaction is not affected by tyrosine phosphorylation of the NPXY motif. Ref.5 Ref.6 Ref.7 Ref.8
Subcellular location: Membrane; Single-pass type I membrane protein. Membrane › coated pit.
Tissue specificity: Absorptive epithelia, including renal proximal tubules.
Involvement in disease: Donnai-Barrow syndrome (DBS) [MIM:222448]: Rare autosomal recessive disorder characterized by major malformations including agenesis of the corpus callosum, congenital diaphragmatic hernia, facial dysmorphology, ocular anomalies, sensorineural hearing loss and developmental delay. The FOAR syndrome was first described as comprising facial anomalies, ocular anomalies, sensorineural hearing loss, and proteinuria. DBS and FOAR were first described as distinct disorders but the classic distinguishing features between the 2 disorders were presence of proteinuria and absence of diaphragmatic hernia and corpus callosum anomalies in FOAR. Early reports noted that the 2 disorders shared many phenotypic features and may be identical. Although there is variability in the expression of some features (e.g., agenesis of the corpus callosum and proteinuria), DBS and FOAR are now considered to represent the same entity.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10
Sequence similarities: Belongs to the LDLR family.Contains 17 EGF-like domains.Contains 36 LDL-receptor class A domains.Contains 37 LDL-receptor class B repeats.
Research Articles on LRP2
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Product Notes
The LRP2 lrp2 (Catalog #AAA690201) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Anti-Human Megalin reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Megalin can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Immunohistochemistry (IHC). IF/IHC: use at 2-5ug/ml. Researchers should empirically determine the suitability of the LRP2 lrp2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Megalin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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