Rabbit anti-Human, Mouse LPXN Polyclonal Antibody | anti-LPXN antibody

LPXN Antibody

Cat. Num. AAA9630688

• Gene Names: LPXN; LDPL
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: LPXN; Polyclonal Antibody; LPXN Antibody; LDLP; LDPL; leupaxin; anti-LPXN antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: LPXN Antibody detects endogenous levels of LPXN.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-LPXN antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human LPXN.
• Conjugation: Unconjugated
• Similarity: The LIM domain 3 is critical for focal adhesion targeting and the suppression of paxillin (PXN) tyrosine phosphorylation. The LIM domain 3 alone or both LIM domains 3 and 4 can mediate interaction with AR. Belongs to the paxillin family.
• Post Translational Modifications: Phosphorylated on tyrosine residues. Phosphorylation on Tyr-72 is important for its inhibitory function. Bombesin stimulates phosphorylation on Tyr-22, Tyr-62 and Tyr-72.
• Subunit Structure: Interacts with PTPN22 (By similarity). Interacts with unphosphorylated ITGA4. Interacts with PTK2B/PYK2, PTPN12, AR and SRF. Interacts (via LD motif 3) with LYN and the interaction is induced upon B-cell antigen receptor (BCR) activation. Interacts (via LD motif 3) with PTK2/FAK.
• Tissue Specificity: Macrophages, monocytes and osteoclasts (at protein level). Strongly expressed in cells and tissues of hematopoietic origin. Highest expression in lymphoid tissues such as spleen, lymph node, thymus and appendix and in the vascular smooth muscle. Lower levels in bone marrow and fetal liver. Also expressed in peripheral blood lymphocytes and a number of hematopoietic cell lines. Very low levels found in epithelial cell lines. Expressed in prostate cancer (PCa) cells and its expression intensity is directly linked to PCa progression.
• Function: Transcriptional coactivator for androgen receptor (AR) and serum response factor (SRF). Contributes to the regulation of cell adhesion, spreading and cell migration and acts as a negative regulator in integrin-mediated cell adhesion events. Suppresses the integrin-induced tyrosine phosphorylation of paxillin (PXN). May play a critical role as an adapter protein in the formation of the adhesion zone in osteoclasts. Negatively regulates B-cell antigen receptor (BCR) signaling.
• Note: huttles between the cytoplasm and nucleus. Recruited to the cell membrane following B-cell antigen receptor (BCR) cross-linking in B-cells. Enhanced focal adhesion kinase activity (PTK2/FAK) attenuates its nuclear accumulation and limits its ability to enhance serum response factor (SRF)-dependent gene transcription. Targeting to focal adhesions is essential for its tyrosine phosphorylation in response to bombesin.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-LPXN antibody

Cytoplasm; Cell junction; Focal adhesion; Perinuclear region; Cell projection; Podosome; Cell membrane; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 8134557
• NCBI GeneID: 9404
• UniProt Accession #: O60711; Q53FW6; Q6FI07; B2R8B4; B4DV71
• Molecular Weight: 43,750 Da
• NCBI Official Full Name: Leupaxin
• NCBI Official Synonym Full Names: leupaxin
• NCBI Official Symbol: LPXN
• NCBI Official Synonym Symbols: LDPL
• NCBI Protein Information: leupaxin
• UniProt Protein Name: Leupaxin
• Protein Family: Leupaxin
• UniProt Gene Name: LPXN
• UniProt Synonym Gene Names: LDLP
• UniProt Entry Name: LPXN_HUMAN

NCBI Description

The product encoded by this gene is preferentially expressed in hematopoietic cells and belongs to the paxillin protein family. Similar to other members of this focal-adhesion-associated adaptor-protein family, it has four leucine-rich LD-motifs in the N-terminus and four LIM domains in the C-terminus. It may function in cell type-specific signaling by associating with PYK2, a member of focal adhesion kinase family. As a substrate for a tyrosine kinase in lymphoid cells, this protein may also function in, and be regulated by, tyrosine kinase activity. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Jan 2009]

Uniprot Description

leupaxin: a LIM domain-containing cytoplasmic protein preferentially expressed in hematopoietic cells. Homologous to the focal adhesion protein, paxillin. Associates with PYK2, a member of focal adhesion kinase family, it may participate in signaling at sites of adhesion. .

Protein type: Adaptor/scaffold; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q12.1

Cellular Component: cell projection; focal adhesion; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; podosome; nucleus

Molecular Function: protein binding; zinc ion binding; transcription cofactor activity

Biological Process: regulation of cell adhesion mediated by integrin; transcription, DNA-dependent; regulation of transcription, DNA-dependent; negative regulation of B cell receptor signaling pathway; protein complex assembly; negative regulation of cell adhesion; signal transduction; cell adhesion