Typical Testing Data/Standard Curve (for reference only)
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Monkey CD27 antigen (CD27) ELISA Kit | CD27 elisa kit
Monkey CD27 antigen (CD27) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
()
Principle of the Assay: CD27 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CD27 antibody and an CD27-HRP conjugate. The assay sample and buffer are incubated together with CD27-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CD27 concentration since CD27 from samples and CD27-HRP conjugate compete for the anti-CD27 antibody binding site. Since the number of sites is limited, as more sites are occupied by CD27 from the sample, fewer sites are left to bind CD27-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CD27 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. [provided by RefSeq, Jul 2008]
Uniprot Description
CD27: Receptor for CD70/CD27L. May play a role in survival of activated T-cells. May play a role in apoptosis through association with SIVA1.
Protein type: Apoptosis; Membrane protein, integral; Receptor, cytokine
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: integral to plasma membrane; extracellular region; external side of plasma membrane
Molecular Function: protein binding; transmembrane receptor activity; caspase inhibitor activity
Biological Process: release of cytoplasmic sequestered NF-kappaB; response to ethanol; cell surface receptor linked signal transduction; positive regulation of JNK cascade; positive regulation of B cell differentiation; immunoglobulin mediated immune response; negative regulation of caspase activity; negative regulation of apoptosis
Disease: Lymphoproliferative Syndrome 2
Research Articles on CD27
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Product Notes
The Monkey CD27 cd27 (Catalog #AAA7246398) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7246398 ELISA Kit recognizes Monkey CD27. It is sometimes possible for the material contained within the vial of "CD27 antigen (CD27), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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