Rabbit anti-Human LOXL2 Polyclonal Antibody | anti-LOXL2 antibody

LOXL2, CT (LOXL2, Lysyl oxidase homolog 2, Lysyl oxidase-like protein 2, Lysyl oxidase-related protein 2, Lysyl oxidase-related protein WS9-14) (FITC)

Cat. Num. AAA6316749

• Gene Names: LOXL2; LOR2; WS9-14
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LOXL2; Polyclonal Antibody; CT (LOXL2; Lysyl oxidase homolog 2; Lysyl oxidase-like protein 2; Lysyl oxidase-related protein 2; Lysyl oxidase-related protein WS9-14) (FITC); Lysyl oxidase-related protein WS9-14; anti-LOXL2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LOXL2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-LOXL2 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 589-617 in the C-terminal region of human LOXL2, UniProt Accession #Q9Y4K0. Species sequence homology: bovine, chicken, mouse, rat
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of A549 cell line lysates (35ug/lane) usingMBS6009602. This demonstrates the LOXL2 antibody detected the LOXL2 protein (arrow).)

Related Product Information for anti-LOXL2 antibody

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family.

Product Categories/Family for anti-LOXL2 antibody

Antibodies; Enzymes; Oxidase

References

Rodriguez, H.M., et al. J. Biol. Chem. 285(27):20964-20974(2010). Ruckert, F., et al. Int J Colorectal Dis 25(3):303-311(2010). Schietke, R., et al. J. Biol. Chem. 285(9):6658-6669(2010). Sano, M., et al. Int. J. Oncol. 36(2):321-330(2010). Kim, Y., et al. Oncol. Rep. 22(4):799-804(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 4505011
• NCBI GeneID: 4017
• NCBI Accession #: NP_002309.1
• NCBI GenBank Nucleotide #: NM_002318.2
• UniProt Accession #: Q9Y4K0; Q53HV3; Q9BW70; Q9Y5Y8; B2R5Q0
• Molecular Weight: 86,725 Da
• NCBI Official Full Name: lysyl oxidase homolog 2
• NCBI Official Synonym Full Names: lysyl oxidase like 2
• NCBI Official Symbol: LOXL2
• NCBI Official Synonym Symbols: LOR2; WS9-14
• NCBI Protein Information: lysyl oxidase homolog 2
• UniProt Protein Name: Lysyl oxidase homolog 2
• Protein Family: Lysyl oxidase
• UniProt Gene Name: LOXL2
• UniProt Entry Name: LOXL2_HUMAN

NCBI Description

This gene encodes a member of the lysyl oxidase gene family. The prototypic member of the family is essential to the biogenesis of connective tissue, encoding an extracellular copper-dependent amine oxidase that catalyses the first step in the formation of crosslinks in collagens and elastin. A highly conserved amino acid sequence at the C-terminus end appears to be sufficient for amine oxidase activity, suggesting that each family member may retain this function. The N-terminus is poorly conserved and may impart additional roles in developmental regulation, senescence, tumor suppression, cell growth control, and chemotaxis to each member of the family. [provided by RefSeq, Jul 2008]

Uniprot Description

LOXL2: Mediates the post-translational oxidative deamination of lysine residues on target proteins leading to the formation of deaminated lysine (allysine). When secreted in extracellular matrix, promotes cross-linking of extracellular matrix proteins by mediating oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Acts as a regulator of sprouting angiogenesis, probably via collagen IV scaffolding. When nuclear, acts as a transcription corepressor and specifically mediates deamination of trimethylated 'Lys-4' of histone H3 (H3K4me3), a specific tag for epigenetic transcriptional activation. Involved in epithelial to mesenchymal transition (EMT) via interaction with SNAI1 and participates in repression of E- cadherin, probably by mediating deamination of histone H3. Also involved in E-cadherin repression following hypoxia, a hallmark of epithelial to mesenchymal transition believed to amplify tumor aggressiveness, suggesting that it may play a role in tumor progression. Acts as a regulator of chondrocyte differentiation, probably by regulating expression of factors that control chondrocyte differentiation. Belongs to the lysyl oxidase family.

Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Oxidoreductase; EC 1.4.3.13

Chromosomal Location of Human Ortholog: 8p21.3

Cellular Component: basement membrane; chromosome; extracellular space; membrane; nucleoplasm; nucleus

Molecular Function: chromatin binding; copper ion binding; electron carrier activity; methylated histone residue binding; protein binding; protein-lysine 6-oxidase activity; scavenger receptor activity; transcription corepressor activity

Biological Process: aging; cell adhesion; collagen fibril organization; endothelial cell migration; endothelial cell proliferation; epithelial to mesenchymal transition; histone modification; negative regulation of transcription, DNA-dependent; positive regulation of chondrocyte differentiation; protein amino acid deamination; protein modification process; receptor-mediated endocytosis; response to copper ion; response to hypoxia; sprouting angiogenesis; transcription, DNA-dependent