Flow Cytometry (FC/FACS)
Rabbit anti-Mouse, Rat LOX 1/Olr1 Polyclonal Antibody | anti-Olr1 antibody
Anti-LOX 1/Olr1 Antibody
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
IHC-P (Embedded Section): 0.5-1ug/ml (Rat)
FC/FACS: 1-3ug/1x106 cells (Rat)
Direct ELISA: 0.1-0.5ug/ml (Rat)
Tested Species: In-house tested species with positive results.
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
Immunohistochemistry (IHC)
Immunohistochemistry (IHC)
Western Blot (WB)
(Figure 1. Western blot analysis of Olr1 using anti-Olr1 antibody.)
Western Blot (WB)
(Figure 1. Western blot analysis of Olr1 using anti-Olr1 antibody.)
Background: OLR1(oxidized low density lipoprotein (lectin-like) receptor 1) also called CLEC8A, LOX-1, SCARE1, is a receptor protein which belongs to the C-type lectin superfamily. The OLR1 gene encodes a cell-surface endocytosis receptor for oxidized low density lipoprotein (OxLDL). This gene is mapped on 12p13.2. Incubation of the cells with LDL had no effect on LOX1 expression, but incubation with OxLDL resulted in a dose-dependent increase in LOX1 mRNA and protein expression; however, very high concentrations of OxLDL caused a decrease in OxLDL expression, perhaps indicating toxic effects on endothelial cells. LOX1 was also expressed in macrophages, but not in vascular smooth muscle cells. The findings suggested a role for LOX1 in the pathophysiology of atherosclerotic cardiovascular disease. LOX1 expression was detected in all choroidal neovascular membranes, regardless of structure, whereas there was no evidence of LOX1 within the posterior segments of normal eyes. LOX1 plays an active role in the pathogenesis of choroidal neovascularization, especially in ARMD.
2. Luedecking-Zimmer, E., DeKosky, S. T., Chen, Q., Barmada, M. M., Kamboh, M. I. Investigation of oxidized LDL-receptor 1 (OLR1) as the candidate gene for Alzheimer's disease on chromosome 12. Hum. Genet. 111: 443-451, 2002.
3. Mango, R., Clementi, F., Borgiani, P., Forleo, G. B., Federici, M., Contino, G., Giardina, E., Garza, L., Fahdi, I. E., Lauro, R., Mehta, J. L., Novelli, G., Romeo, F. Association of single nucleotide polymorphisms in the oxidised LDL receptor 1 (OLR1) gene in patients with acute myocardial infarction. (Letter) J. Med. Genet. 40: 933-936, 2003.
NCBI and Uniprot Product Information
NCBI Description
receptor for oxidized low-density lipoprotein that may be involved in pathogenesis of hypertension as well as atherosclerosis [RGD, Feb 2006]
Uniprot Description
Function: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria. Ref.2 Ref.4
Subunit structure: Homodimer; disulfide-linked. May form a hexamer composed of 3 homodimers. Interacts with HSP70
By similarity.
Subcellular location: Cell membrane; Lipid-anchor
By similarity. Cell membrane; Single-pass type II membrane protein
By similarity. Membrane raft
By similarity. Secreted
By similarity. Note: A secreted form also exists. Localization to membrane rafts requires palmitoylation
By similarity.
Tissue specificity: Predominantly expressed in lung and at lower level in kidney. Expressed in macrophages but not in vascular smooth muscle cells. Ref.1
Induction: By hypertension. Up-regulated by shear stress, lipopolysaccharide and TNF-alpha in cultured vascular endothelial cells. Ref.1 Ref.2
Domain: The cytoplasmic region is required for subcellular sorting on the cell surface
By similarity.The C-type lectin domain mediates the recognition and binding of oxLDL
By similarity.The Neck region contains 3 internal repeats that are only found in rodents.
Post-translational modification: N-glycosylated
By similarity.
Sequence similarities: Contains 1 C-type lectin domain.
Research Articles on Olr1
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Product Notes
The Olr1 olr1 (Catalog #AAA1752952) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LOX 1/Olr1 Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LOX 1/Olr1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Direct ELISA (EIA). WB: 0.25-0.5ug/ml (Mouse, Rat) IHC-P (Embedded Section): 0.5-1ug/ml (Rat) FC/FACS: 1-3ug/1x106 cells (Rat) Direct ELISA: 0.1-0.5ug/ml (Rat) Tested Species: In-house tested species with positive results. Researchers should empirically determine the suitability of the Olr1 olr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LOX 1/Olr1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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