Rabbit anti-E Coli LON PROTEASE Polyclonal Antibody | anti-LONP1 antibody

Anti-LON PROTEASE Antibody, Rabbit Polyclonal

Cat. Num. AAA8110632

• Gene Names: lon; capR; deg; dir; ECK0433; JW0429; lonA; lopA; muc
• Reactivity: E Coli
• Applications: ELISA
• Purity: Protein A
• Synonyms: LON PROTEASE; Polyclonal Antibody; Anti-LON PROTEASE Antibody; Rabbit Polyclonal; Escherichia coli LON PROTEASE Antibody; Rabbit PAb; anti-LONP1 antibody

• Host: Rabbit
• Reactivity: E Coli
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Escherichia coli LON PROTEASE
• Purity/Purification: Protein A
• Form/Format: 0.2um filtered solution in PBS

• Applicable Applications for anti-LONP1 antibody: ELISA (EIA)
• Application Notes: ELISA: 1:1000-1:2000; This antibody can be used at 1:100-1:2000 with the appropriate secondary reagents to detect E.coli LON PROTEASE.
• Immunogen: Recombinant Escherichia coli LON PROTEASE protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Escherichia coli LON PROTEASE. Total IgG was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Related Product Information for anti-LONP1 antibody

Lon protease, an ATP-dependent mitochondrial protease, is important in mitochondrial protein maintenance. Lon protease is a multifunctional enzyme, and its functions include the degradation of damaged proteins and naturally short-lived proteins, ATPase and chaperone-like activities, as well as DNA binding. Lon protease plays a major role in the protein quality control system in mammalian cell mitochondria. It is present in the mitochondrial matrix and degrades oxidized and misfolded proteins, thereby protecting the cell from various extracellular stresses, including oxidative stress. The intellectual disability-associated and thalidomide-binding protein cereblon (CRBN) contains a large, highly conserved Lon domain. The Lon ATP-dependent protease plays an important role in regulating many biological processes in bacteria.

NCBI and Uniprot Product Information

• NCBI GI #: 16128424
• NCBI GeneID: 945085
• NCBI Accession #: NP_414973.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P0A9M0; P08177; P78219; Q2MBY7
• NCBI Official Full Name: DNA-binding ATP-dependent protease La
• NCBI Official Symbol: lon
• NCBI Official Synonym Symbols: capR; deg; dir; ECK0433; JW0429; lonA; lopA; muc
• NCBI Protein Information: DNA-binding ATP-dependent protease La
• UniProt Protein Name: Lon protease
• Protein Family: Lon protease
• UniProt Gene Name: lon

NCBI Description

Lon dependent lethality is due to induction of yefM-yoeb toxin/antitoxin pair. [More information is available at EcoGene: EG10542]. Lon is an ATP-dependent protease responsible for degradation of misfolded proteins as well as a number of rapidly degraded regulatory proteins. [More information is available at EcoCyc: EG10542].

Uniprot Description

ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins, including some antitoxins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner. Endogenous substrates include the regulatory proteins RcsA and SulA, the transcriptional activator SoxS, UmuD and at least type II antitoxins CcdA, HipB and MazE (PubMed:8022284, PubMed:16460757, PubMed:22720069, PubMed:24375411). Its overproduction specifically inhibits translation through at least two different pathways, one of them being the YoeB-YefM toxin-antitoxin system (PubMed:15009896). Overproduction leads to very high persister cell formation (cells highly resistant to antibiotics), but not when 10 mRNA interferases are deleted, nor when Lon catalytic residues are mutated (PubMed:21788497).