Western Blot (WB)
(Western blot analysis of LMX1B expression in HeLa (A), Jurkat (B), SP2/0 (C) whole cell lysates.)
Rabbit LMX1B Polyclonal Antibody | anti-LMX1B antibody
Anti-LMX1B Antibody
Western Blot (WB)
(Western blot analysis of LMX1B expression in HeLa (A), Jurkat (B), SP2/0 (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of LMX1B expression in HeLa (A), Jurkat (B), SP2/0 (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of LMX1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of LMX1B staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of LIM-homeodomain family of proteins containing two N-terminal zinc-binding LIM domains, 1 homeodomain, and a C-terminal glutamine-rich domain. It functions as a transcription factor, and is essential for the normal development of dorsal limb structures, the glomerular basement membrane, the anterior segment of the eye, and dopaminergic and serotonergic neurons. Mutations in this gene are associated with nail-patella syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
Uniprot Description
LMX1B: Essential for the specification of dorsal limb fate at both the zeugopodal and autopodal levels. Defects in LMX1B are the cause of nail-patella syndrome (NPS); also known as onychoosteodysplasia. NPS is a disease that cause abnormal skeletal patterning and renal dysplasia. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cell development/differentiation; Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 9q33.3
Cellular Component: nucleus
Molecular Function: protein binding; zinc ion binding; sequence-specific DNA binding; transcription factor activity
Biological Process: collagen fibril organization; transcription, DNA-dependent; in utero embryonic development; multicellular organismal development; neuron migration; cerebellum morphogenesis; limb morphogenesis; neuron differentiation; cell proliferation; organ growth; dorsal/ventral pattern formation; regulation of transcription, DNA-dependent; midbrain development; positive regulation of transcription from RNA polymerase II promoter; central nervous system neuron development
Disease: Nail-patella Syndrome
Research Articles on LMX1B
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Product Notes
The LMX1B lmx1b (Catalog #AAA822256) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LMX1B Antibody reacts with Human, Bovine, Dog, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's LMX1B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP). WB (1/500 - 1/1000), IH (1/100 - 1/200), IP (1/10 - 1/100). Researchers should empirically determine the suitability of the LMX1B lmx1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LMX1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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