Immunofluorescence (IF)
(MBS9604865 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Rabbit anti-Human, Mouse LMTK3 Polyclonal Antibody | anti-LMTK3 antibody
LMTK3 Antibody
Gene Names
LMTK3; LMR3; TYKLM3; PPP1R101
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
LMTK3; Polyclonal Antibody; LMTK3 Antibody; BC059845; KIAA1883; Lemur tyrosine kinase 3; LMR3; MGC90743; Serine/threonine-protein kinase LMTK3; TYKLM3; anti-LMTK3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
LMTK3 antibody detects endogenous levels of total LMTK3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
1460
Applicable Applications for anti-LMTK3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:1000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide
Subcellular Location
Cell Membrane
Predicted Cross Reactivity
Pig, Bovine
Similarity
Pig (100%), Bovine (100%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9604865 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9604865 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using LMTK3 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from 293 cells, using LMTK3 antibody.)
Related Product Information for anti-LMTK3 antibody
Function: Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription (PubMed:21602804). Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons (By similarity).
Subunit Structure: Interacts with ESR1 (PubMed:21602804). Interacts with AP-2 complex subunit alpha (By similarity).
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family.
Subunit Structure: Interacts with ESR1 (PubMed:21602804). Interacts with AP-2 complex subunit alpha (By similarity).
Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Observed: 40 kD
Predicted: 154 kDa
Predicted: 154 kDa
NCBI Official Full Name
Serine/threonine-protein kinase LMTK3
NCBI Official Synonym Full Names
lemur tyrosine kinase 3
NCBI Official Symbol
LMTK3
NCBI Official Synonym Symbols
LMR3; TYKLM3; PPP1R101
NCBI Protein Information
serine/threonine-protein kinase LMTK3
UniProt Protein Name
Serine/threonine-protein kinase LMTK3
Protein Family
UniProt Gene Name
LMTK3
UniProt Synonym Gene Names
KIAA1883; TYKLM3
Uniprot Description
Protein kinase which phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. May also regulate ESR1 levels indirectly via a PKC-AKT-FOXO3 pathway where it decreases the activity of PKC and the phosphorylation of AKT, thereby increasing binding of transcriptional activator FOXO3 to the ESR1 promoter and increasing ESR1 transcription (PubMed:21602804). Involved in endocytic trafficking of N-methyl-D-aspartate receptors (NMDAR) in neurons ().
Research Articles on LMTK3
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Product Notes
The LMTK3 lmtk3 (Catalog #AAA9604865) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LMTK3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LMTK3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:1000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the LMTK3 lmtk3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LMTK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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