Sheep Lctin like oxLDL Receptor 1 ELISA Kit | LOX-1 elisa kit

Sheep Lctin like oxLDL Receptor 1 ELISA Kit

Cat. Num. AAA737059

• Gene Names: Olr1; LOX-1; Oldr1; Oldlr1
• Reactivity: Sheep
• Synonyms: Lctin like oxLDL Receptor 1; Sheep Lctin like oxLDL Receptor 1 ELISA Kit; LOX-1 elisa kit

• Reactivity: Sheep

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This LOX-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Sheep LOX-1. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LOX-1 elisa kit

Principle of the assay: LOX-1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-LOX-1 antibody and an LOX-1-HRP conjugate. The assay sample and buffer are incubated together with LOX-1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the LOX-1 concentration since LOX-1 from samples and LOX-1-HRP conjugate compete for the anti-LOX-1 antibody binding site. Since the number of sites is limited, as more sites are occupied by LOX-1 from the sample, fewer sites are left to bind LOX-1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The LOX-1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4008187
• NCBI GeneID: 140914
• Molecular Weight: 41,890 Da
• NCBI Official Full Name: lectin-like oxidized low-density lipoprotein receptor
• NCBI Official Synonym Full Names: oxidized low density lipoprotein (lectin-like) receptor 1
• NCBI Official Symbol: Olr1
• NCBI Official Synonym Symbols: LOX-1; Oldr1; Oldlr1
• NCBI Protein Information: oxidized low-density lipoprotein receptor 1; ox-LDL receptor 1; lectin-like oxLDL receptor 1; lectin-like oxidized LDL receptor 1; lectin-type oxidized LDL receptor 1; lectin-like oxidized low-density lipoprotein receptor; Lectin-like oxidized low-density lipoprotein receptor-1; oxidised low density lipoprotein (lectin-like) receptor 1
• UniProt Protein Name: Oxidized low-density lipoprotein receptor 1
• UniProt Gene Name: Olr1
• UniProt Synonym Gene Names: Lox1; Oldlr1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1
• UniProt Entry Name: OLR1_RAT

NCBI Description

receptor for oxidized low-density lipoprotein that may be involved in pathogenesis of hypertension as well as atherosclerosis [RGD, Feb 2006]

Uniprot Description

Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria.