Rabbit anti-Human Lipoxygenase, 5- Polyclonal Antibody | anti-ALOX5 antibody

Lipoxygenase, 5- (5-LOX, 5-LO, 5-LPG, Arachidonate 5-Lipoxygenase, Arachidonic Acid 5-Lipoxygenase, ALOX5, Leukotriene A4 Synthase, LOG5, MGC163204)

Cat. Num. AAA623504

• Gene Names: ALOX5; 5-LO; 5LPG; LOG5; 5-LOX
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Lipoxygenase; 5-; Polyclonal Antibody; 5- (5-LOX; 5-LO; 5-LPG; Arachidonate 5-Lipoxygenase; Arachidonic Acid 5-Lipoxygenase; ALOX5; Leukotriene A4 Synthase; LOG5; MGC163204); Anti -Lipoxygenase; anti-ALOX5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Species Crossreactivity: This antibody reacts with the human protein.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: PBS + 30% glycerol

• Applicable Applications for anti-ALOX5 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.
• Immunogen: A synthetic peptide made to a region within residues 100-200 of the human protein. [Swiss-Prot# P09917]. Homology: Human 100% Mouse 95% Hamster 95%
• Cellular Localization: Cytoplasmic
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ALOX5 antibody

The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.The enzyme 5-lipoxygenase (5-LO) plays a key role in regulating the production of leukotrienes (LTs) (Funk, 2001). Overproduction of LTs contributes to several diseases, most notably chronic inflammatory diseases, including asthma (Drazen et al., 1994), fibrosis (Wilborn et al., 1996) and atherosclerosis (Dwyer et al., 2004). Recent work has demonstrated that the activity of 5-LO is regulated by PKA phosphorylation of serine-523 in 5-LO (Luo et al., 2004). Under normal conditions, this phosphorylation may be important in limiting inflammation. Abnormal signaling through cAMP and PKA, then, could contribute to a variety of diseases, including those characterized by chronic inflammation. The phospho-specific antibody to Ser523 on 5-LO is thus likely to provide a valuable tool for studies of the role of 5-LO regulation in diseases such as asthma, fibrosis and atherosclerosis.

Product Categories/Family for anti-ALOX5 antibody

Antibodies; Abs to Leukotrienes

NCBI and Uniprot Product Information

• NCBI GI #: 187193
• NCBI GeneID: 240
• UniProt Accession #: P09917; Q5JQ14; B7ZLS0
• Molecular Weight: 77,983 Da
• NCBI Official Full Name: lipoxygenase
• NCBI Official Synonym Full Names: arachidonate 5-lipoxygenase
• NCBI Official Symbol: ALOX5
• NCBI Official Synonym Symbols: 5-LO; 5LPG; LOG5; 5-LOX
• NCBI Protein Information: arachidonate 5-lipoxygenase; LOX-5; leukotriene A4 synthase; arachidonic acid 5-lipoxygenase; arachidonic 5-lipoxygenase alpha-10 isoform; arachidonic 5-lipoxygenase delta-13 isoform; arachidonic 5-lipoxygenase delta-p10 isoform; arachidonic 5-lipoxygenase delta-10-13 isoform
• UniProt Protein Name: Arachidonate 5-lipoxygenase
• Protein Family: Arachidonate 5-lipoxygenase
• UniProt Gene Name: ALOX5
• UniProt Synonym Gene Names: LOG5; 5-LO
• UniProt Entry Name: LOX5_HUMAN

NCBI Description

This gene encodes a member of the lipoxygenase gene family and plays a dual role in the synthesis of leukotrienes from arachidonic acid. The encoded protein, which is expressed specifically in bone marrow-derived cells, catalyzes the conversion of arachidonic acid to 5(S)-hydroperoxy-6-trans-8,11,14-cis-eicosatetraenoic acid, and further to the allylic epoxide 5(S)-trans-7,9-trans-11,14-cis-eicosatetrenoic acid (leukotriene A4). Leukotrienes are important mediators of a number of inflammatory and allergic conditions. Mutations in the promoter region of this gene lead to a diminished response to antileukotriene drugs used in the treatment of asthma and may also be associated with atherosclerosis and several cancers. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

5-LO: arachidonate 5-lipoxygenase. Arachidonic acid promotes its phosphorylation by p38 MAPK-regulated MAPKAP kinases. Upregulated in glioblastoma multiforme and colon, pancreatic and bladder cancer.

Protein type: Nuclear envelope; Lipid Metabolism - linoleic acid; EC 1.13.11.34; Lipid Metabolism - arachidonic acid; Oxidoreductase

Chromosomal Location of Human Ortholog: 10q11.2

Cellular Component: extracellular space; nuclear membrane; nuclear matrix; nuclear envelope lumen; nuclear envelope; cytosol

Molecular Function: protein binding; iron ion binding; arachidonate 5-lipoxygenase activity

Biological Process: leukotriene biosynthetic process; leukotriene production during acute inflammatory response; lipoxygenase pathway; arachidonic acid metabolic process; leukotriene metabolic process

Disease: Asthma, Susceptibility To