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Western Blot (WB) (Western Blot analysis of extracts of mouse testis cells, using LIPG antibody.)

Rabbit LIPG Polyclonal Antibody | anti-LIPG antibody

LIPG Polyclonal Antibody

Gene Names
LIPG; EL; EDL; PRO719
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
LIPG; Polyclonal Antibody; LIPG Polyclonal Antibody; EDL; EL; Endothelial cell-derived lipase; Endothelial lipase; Endothelial lipase precursor; Lipase endothelial; LIPE_HUMAN; Lipoprotein lipase H; PL; PRO719; anti-LIPG antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
LIPG polyclonal antibody detects endogenous levels of LIPG protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
500
Applicable Applications for anti-LIPG antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
Immunogen
Recombinant full length Human LIPG.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot analysis of extracts of mouse testis cells, using LIPG antibody.)

Western Blot (WB) (Western Blot analysis of extracts of mouse testis cells, using LIPG antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded rat kidney using LIPG antibody at dilution of 1:200 (400x lens).)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat kidney using LIPG antibody at dilution of 1:200 (400x lens).)
Related Product Information for anti-LIPG antibody
The Lipase gene family belongs to one of the most robust genetic superfamilies found in living organisms, which includes esterases and thioesterases. Members of the AB hydrolase subfamily include hepatic lipase (HL), endothelial lipase (EDL or EL), lipoprotein lipase (LPL), pancreatic lipase (PL), gastric lipase (GL) and LCAT. These family members play a crucial role in the metabolism of lipids. Defects in LPL may cause chylomicronemia syndrome or a form of lipoprotein lipase deficiency characterized by hypertriglyceridemia. Endothelial lipase, which also is known as endothelial cell-derived lipase, has both triglyceride and phospholipase activity. This protein, which is synthesized in endothelial cells, can bind heparin. It is expressed primarily in placenta, liver, thyroid, kidney, lung, testis and ovary tissue.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 57kDa
NCBI Official Full Name
endothelial lipase isoform 1
NCBI Official Synonym Full Names
lipase G, endothelial type
NCBI Official Symbol
LIPG
NCBI Official Synonym Symbols
EL; EDL; PRO719
NCBI Protein Information
endothelial lipase
UniProt Protein Name
Endothelial lipase
Protein Family
UniProt Gene Name
LIPG
UniProt Synonym Gene Names
EDL; EL
UniProt Entry Name
LIPE_HUMAN

NCBI Description

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]

Uniprot Description

LIPG: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - glycerolipid; Phospholipase; Secreted; EC 3.1.1.3; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 18q21.1

Cellular Component: Golgi apparatus; extracellular space; cell surface; early endosome

Molecular Function: heparin binding; lipoprotein lipase activity; phospholipase A1 activity; phospholipase activity

Biological Process: cell proliferation; cholesterol homeostasis; reverse cholesterol transport; phospholipid catabolic process; regulation of lipoprotein metabolic process; lipid metabolic process; positive regulation of cholesterol transport; response to nutrient; phospholipid homeostasis

Research Articles on LIPG

Similar Products

Product Notes

The LIPG lipg (Catalog #AAA3011245) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LIPG Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LIPG can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the LIPG lipg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIPG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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