Typical Testing Data/Standard Curve (for reference only)
Human lipase, endothelial ELISA Kit | LIPG elisa kit
Human Endothelial lipase, EL ELISA Kit
Gene Names
LIPG; EL; EDL; PRO719
Reactivity
Human
Synonyms
lipase; endothelial; Human Endothelial lipase; EL ELISA Kit; EDL; EL; PRO719; endothelial cell-derived lipase; lipase G; lipoprotein lipase H; LIPG elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Human LIPG. No significant cross-reactivity or interference between Human LIPG and analogues was observed.
Sequence Length
500
Samples
Serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range
0.625 ng/ml-40 ng/ml
Sensitivity
0.156 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV% is less than 8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV% is less than 10%
Three samples of known concentration were tested in twenty assays to assess.
Detection Wavelength
450 nm
Sample Volume
50-100ul
Protein Biological Process 1
Biosynthesis/Metabolism
Protein Biological Process 2
Lipogenesis and lipometabolism
Protein Biological Process 3
Lipid degradation
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for LIPG elisa kit
Introduction: Endothelial lipase (EL) is a newly described member of the triglyceride lipase gene family. It has a considerable molecular homology with lipoprotein lipase (LPL) (44%) and hepatic lipase (HL) (41%). Unlike LPL and HL, this enzyme is synthesized by endothelial cells and functions at the site where it is synthesized. Furthermore, its tissue distribution is different from that of LPL and HL. Unlike LPL and HL, EL is located in the vascular endothelial cells and its expression is highly regulated by cytokines and physical forces, suggesting that it may play a role in the development of atherosclerosis. As a lipase, EL has primarily phospholipase A1 activity. Animals that overexpress EL showed reduced HDL cholesterol levels. Conversely, animals that are deficient in EL showed a marked elevation in HDL cholesterol levels, suggesting that it is a major genetic determinant for the concentration, structure, and metabolism of HDL, which protects against atherosclerosis.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for EL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain EL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of EL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to EL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for EL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain EL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of EL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,795 Da
NCBI Official Full Name
endothelial lipase
NCBI Official Synonym Full Names
lipase, endothelial
NCBI Official Symbol
LIPG
NCBI Official Synonym Symbols
EL; EDL; PRO719
NCBI Protein Information
endothelial lipase; lipoprotein lipase H; endothelial cell-derived lipase
UniProt Protein Name
Endothelial lipase
Protein Family
UniProt Gene Name
LIPG
UniProt Synonym Gene Names
UNQ387/PRO719; EDL; EL
UniProt Entry Name
LIPE_HUMAN
NCBI Description
The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq, Jul 2008]
Uniprot Description
Function: Has phospholipase and triglyceride lipase activities. Hydrolyzes high density lipoproteins (HDL) more efficiently than other lipoproteins. Binds heparin.
Catalytic activity: Triacylglycerol + H2O = diacylglycerol + a carboxylate.
Enzyme regulation: Inhibited by serum.
Research Articles on LIPG
Similar Products
Product Notes
The Human LIPG lipg (Catalog #AAA702323) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA702323 ELISA Kit recognizes Human LIPG. It is sometimes possible for the material contained within the vial of "lipase, endothelial, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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