Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Lipase A Polyclonal Antibody | anti-LIPA antibody
Polyclonal Antibody to Lipase A, Lysosomal Acid (LIPA)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NMNVTEIIM RWGYPGEEHS VLTGDGYILS IHRIPRGRKN HFGKGPRPVV YLQHGLLADS SNWVTNIDNS SLGFLLADAG FDVWMGNSRG NTWSLKHKTL SVSQDEFWAF SFDEMAKYDL PASINYILNK TGQEQIYYVG
Immunohistochemistry: 5-20ug/mL;1:40-160
Immunocytochemistry: 5-20ug/mL;1:40-160
Optimal working dilutions must be determined by end user.
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Liver tissue))
NCBI and Uniprot Product Information
Uniprot Description
LIPA: Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor- mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation. Defects in LIPA are the cause of Wolman disease (WOD). WOD is a severe manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. WOD occurs in infancy and is nearly always fatal before the age of 1 year. Defects in LIPA are the cause of cholesteryl ester storage disease (CESD). CESD is a mild manifestation of LIPA deficiency, leading to the accumulation of cholesteryl esters and triglycerides in most tissues of the body. It is characterized by late-onset. Belongs to the AB hydrolase superfamily. Lipase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.1.1.13; Hydrolase; Lipid Metabolism - steroid biosynthesis
Chromosomal Location of Human Ortholog: 19|19 C1
Cellular Component: fibrillar center; intracellular membrane-bound organelle; lysosome
Molecular Function: hydrolase activity; hydrolase activity, acting on ester bonds; lipase activity; sterol esterase activity
Biological Process: cell morphogenesis; cell proliferation; cytokine production; fatty acid metabolic process; homeostasis of number of cells within a tissue; inflammatory response; lipid catabolic process; lipid metabolic process; lung development; sterol metabolic process; tissue remodeling
Research Articles on LIPA
Similar Products
Product Notes
The LIPA lipa (Catalog #AAA2027334) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Lipase A, Lysosomal Acid (LIPA) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Lipase A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/mL;1:400-1600 Immunohistochemistry: 5-20ug/mL;1:40-160 Immunocytochemistry: 5-20ug/mL;1:40-160 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the LIPA lipa for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NMNVTEIIM RWGYPGEEHS VLTGDGYILS IHRIPRGRKN HFGKGPRPVV YLQHGLLADS SNWVTNIDNS SLGFLLADAG FDVWMGNSRG NTWSLKHKTL SVSQDEFWAF SFDEMAKYDL PASINYILNK TGQEQIYYVG. It is sometimes possible for the material contained within the vial of "Lipase A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
