Immunofluorescence (IF)
(MBS9600400 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Rabbit anti-Human, Mouse AKAP3 Polyclonal Antibody | anti-AKAP3 antibody
AKAP3 Antibody
Gene Names
AKAP3; CT82; SOB1; FSP95; PRKA3; HEL159; AKAP110; AKAP 110
Reactivity
Human, Mouse
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
AKAP3; Polyclonal Antibody; AKAP3 Antibody; A kinase (PRKA) anchor protein 3; A-kinase anchor protein 110 kDa; A-kinase anchor protein 3; AKAP 110; AKAP-3; AKAP110; Akap3; AKAP3_HUMAN; Cancer/testis antigen 82; CT82; Fibrous sheath protein of 95 kDa; Fibrousheathin 1; Fibrousheathin I; Fibrousheathin-1; FSP95; PRKA3; protein kinase A binding protein AKAP 110; Protein kinase A-anchoring protein 3; SOB 1; SOB1; Sperm oocyte binding protein 1; Sperm oocyte-binding protein; anti-AKAP3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
AKAP3 antibody detects endogenous levels of AKAP3
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
853
Applicable Applications for anti-AKAP3 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
IF/ICC: 1:100-1:500
WB: 1:500-1:2000
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human AKAP3
Tissue Specificity
Testis specific; only expressed in spermatids.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunofluorescence (IF)
(MBS9600400 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunofluorescence (IF)
(MBS9600400 staining HeLa cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2, using AKAP3 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of extracts from HepG2, using AKAP3 Antibody. Lane 1 was treated with the antigen-specific peptide.)
Related Product Information for anti-AKAP3 antibody
Description: May function as a regulator of both motility-and head-associated functions such as capacitation and the acrosome reaction.
Subunit Structure: Interacts with ROPN1 AND ROPN1L.
Post-translational Modifications: Phosphorylated on tyrosine residues.
Similarity: RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer. Belongs to the AKAP110 family.
Subunit Structure: Interacts with ROPN1 AND ROPN1L.
Post-translational Modifications: Phosphorylated on tyrosine residues.
Similarity: RII-binding site, predicted to form an amphipathic helix, could participate in protein-protein interactions with a complementary surface on the R-subunit dimer. Belongs to the AKAP110 family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 94 kDa
Predicted: 95 kDa
Predicted: 95 kDa
NCBI Official Full Name
A-kinase anchor protein 3
NCBI Official Synonym Full Names
A-kinase anchoring protein 3
NCBI Official Symbol
AKAP3
NCBI Official Synonym Symbols
CT82; SOB1; FSP95; PRKA3; HEL159; AKAP110; AKAP 110
NCBI Protein Information
A-kinase anchor protein 3
UniProt Protein Name
A-kinase anchor protein 3
Protein Family
UniProt Gene Name
AKAP3
UniProt Synonym Gene Names
AKAP110; SOB1; AKAP-3; AKAP 110; CT82; FSP95; PRKA3
NCBI Description
This gene encodes a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. The encoded protein is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. This protein is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction. [provided by RefSeq, May 2013]
Uniprot Description
May function as a regulator of both motility- and head-associated functions such as capacitation and the acrosome reaction.
Research Articles on AKAP3
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Product Notes
The AKAP3 akap3 (Catalog #AAA9600400) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The AKAP3 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's AKAP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). IF/ICC: 1:100-1:500 WB: 1:500-1:2000. Researchers should empirically determine the suitability of the AKAP3 akap3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "AKAP3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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