Mouse Lipase A, Lysosomal Acid (LIPA) ELISA Kit | LIPA elisa kit

Mouse Lipase A, Lysosomal Acid (LIPA) ELISA Kit

Cat. Num. AAA455490

• Gene Names: Lipa; Lal; Lip1; Lip-1; AA960673
• Reactivity: Mouse
• Synonyms: Lipase A; Lysosomal Acid (LIPA); Mouse Lipase A; Lysosomal Acid (LIPA) ELISA Kit; LAL; CESD; Lysosomal Acid; Cholesterol Esterase(Wolman Disease); Acid Cholesteryl Ester Hydrolase; Lysosomal acid lipase/cholesteryl ester hydrolase; LIPA elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of LIPA. No significant cross-reactivity or interference between LIPA and analogues was observed.
• Sequence Length: 397

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.117ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LIPA were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LIPA were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LIPA elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of LIPA in mouse serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LIPA. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LIPA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LIPA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LIPA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 162287343
• NCBI GeneID: 16889
• NCBI Accession #: NP_001104570.1
• NCBI GenBank Nucleotide #: NM_001111100.1
• UniProt Accession #: Q9Z0M5; Q8C2G7
• Molecular Weight: 45,325 Da
• NCBI Official Full Name: lysosomal acid lipase/cholesteryl ester hydrolase
• NCBI Official Synonym Full Names: lysosomal acid lipase A
• NCBI Official Symbol: Lipa
• NCBI Official Synonym Symbols: Lal; Lip1; Lip-1; AA960673
• NCBI Protein Information: lysosomal acid lipase/cholesteryl ester hydrolase
• UniProt Protein Name: Lysosomal acid lipase/cholesteryl ester hydrolase
• Protein Family: Lipoprotein
• UniProt Gene Name: Lipa
• UniProt Synonym Gene Names: Lip1; Acid cholesteryl ester hydrolase; LAL

Uniprot Description

Crucial for the intracellular hydrolysis of cholesteryl esters and triglycerides that have been internalized via receptor-mediated endocytosis of lipoprotein particles. Important in mediating the effect of LDL (low density lipoprotein) uptake on suppression of hydroxymethylglutaryl-CoA reductase and activation of endogenous cellular cholesteryl ester formation ().