Rabbit LIN7A Polyclonal Antibody | anti-LIN7A antibody

Anti-LIN7A Antibody, Rabbit Polyclonal

Cat. Num. AAA8103674

• Gene Names: LIN7A; LIN7; VELI1; LIN-7A; MALS-1; TIP-33
• Reactivity: Human; Predicted to React with: Mouse, Rat, Bovin (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: LIN7A; Polyclonal Antibody; Anti-LIN7A Antibody; Rabbit Polyclonal; LIN7A Antibody; Rabbit PAb; Antigen Affinity Purified; lin-7 homolog A (C. elegans); Anti-LIN-7A Antibody; Anti-LIN7 Antibody; Anti-MALS-1 Antibody; Anti-TIP-33 Antibody; Anti-VELI1 Antibody; anti-LIN7A antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat, Bovin (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human LIN7A
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LIN7A antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; ICC/IF: 1:300-1:10,000; IP: 5-10uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the center region of the Human LIN7A
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human LIN7A, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-LIN7A rabbit polyclonal antibody at 1:500 dilution Lane A: Mouse brain tissue lysate Lane B: Rat brain tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:26 kDa)

Immunofluorescence (IF)

(Immunofluorescence staining of LIN7A in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human LIN7A polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and cell membrane.)

Immunoprecipitation (IP)

(ATP6V1C1 was immunoprecipitated using: Lane A:0.5 mg HepG2 Whole Cell Lysate 4 uL anti-ATP6V1C1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G. Primary antibody: Anti-ATP6V1C1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody: Clean-Blot IP Detection Reagent (HRP) at 1:1000dilution Developed using the ECL technique. Performed under reducing conditions. Predicted band size: 44 kDa Observed band size :45 kDa)

Related Product Information for anti-LIN7A antibody

LIN7A (Lin-7 Homolog A, Crumbs Cell Polarity Complex Component) is a Protein Coding gene. LIN7A encodes a scaffold protein that is important for synaptic function, as a possible responsible gene for ID. The encoded protein also is required for the localization of some specific channels and can be part of a protein complex that couples synaptic vesicle exocytosis to cell adhesion in the brain. LIN7A is a candidate gene responsible for 12q21-deletion syndrome, and abnormal neuronal migration and interhemispheric axon development may contribute to ID and corpus callosum hypoplasia, respectively. LIN7A has a crucial role in the polarity abnormalities associated with breast carcinogenesis. Diseases associated with LIN7A include Median Arcuate Ligament Syndrome and Pulmonary Aspergilloma.

NCBI and Uniprot Product Information

• NCBI GI #: 4759306
• NCBI GeneID: 8825
• NCBI Accession #: NP_004655.1
• NCBI GenBank Nucleotide #: NM_004664.2
• UniProt Accession #: O14910; Q147W1; Q6LES3; Q7LDS4; A4FTY3
• Molecular Weight: 25,997 Da
• NCBI Official Full Name: protein lin-7 homolog A
• NCBI Official Synonym Full Names: lin-7 homolog A (C. elegans)
• NCBI Official Symbol: LIN7A
• NCBI Official Synonym Symbols: LIN7; VELI1; LIN-7A; MALS-1; TIP-33
• NCBI Protein Information: protein lin-7 homolog A; hLin-7; mammalian LIN-7 1; mammalian lin-seven protein 1; tax interaction protein 33; veli-1; vertebrate LIN7 homolog 1; vertebrate lin-7 homolog 1
• UniProt Protein Name: Protein lin-7 homolog A
• Protein Family: Protein
• UniProt Gene Name: LIN7A
• UniProt Synonym Gene Names: MALS1; VELI1; Lin-7A; hLin-7; MALS-1; TIP-33; Veli-1
• UniProt Entry Name: LIN7A_HUMAN

Uniprot Description

LIN7A: Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. Belongs to the lin-7 family.

Protein type: Adaptor/scaffold; Cell adhesion

Chromosomal Location of Human Ortholog: 12q21

Cellular Component: postsynaptic membrane; neuron projection; tight junction; basolateral plasma membrane; postsynaptic density; synapse; intercellular junction

Molecular Function: protein binding; PDZ domain binding

Biological Process: asymmetric protein localization; protein transport; exocytosis; neurotransmitter secretion; synaptic vesicle transport; protein complex assembly; inner ear development; maintenance of epithelial cell polarity