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Western Blot (WB) (Western blotAll lanes: LIMS1 antibody at 0.93ug/ml+A549 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 38,39,45,42 kDaObserved band size: 37 kDa)

Rabbit anti-Human LIMS1 Polyclonal Antibody | anti-LIMS1 antibody

LIMS1 Antibody

Gene Names
LIMS1; PINCH; PINCH1; PINCH-1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
LIMS1; Polyclonal Antibody; LIMS1 Antibody; LIM and senescent cell antigen-like-containing domain protein 1; Particularly interesting new Cys-His protein 1; PINCH-1; Renal carcinoma antigen NY-REN-48; PINCH; PINCH1; anti-LIMS1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
329
Applicable Applications for anti-LIMS1 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Species
Human
Immunogen
Recombinant human LIM and senescent cell antigen-like-containing domain protein 1 protein (1-325AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-47912 / sc-47915 / sc-47914 / sc-67177
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: LIMS1 antibody at 0.93ug/ml+A549 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 38,39,45,42 kDaObserved band size: 37 kDa)

Western Blot (WB) (Western blotAll lanes: LIMS1 antibody at 0.93ug/ml+A549 whole cell lysateSecondaryGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 38,39,45,42 kDaObserved band size: 37 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human colon cancer using MBS7045748 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human colon cancer using MBS7045748 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7045748 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human kidney tissue using MBS7045748 at dilution 1:100)

Immunofluorescence (IF)

(Immunofluorescent analysis of Hela cells using MBS7045748 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))

Immunofluorescence (IF) (Immunofluorescent analysis of Hela cells using MBS7045748 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Related Product Information for anti-LIMS1 antibody
Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
41,571 Da
NCBI Official Full Name
LIM and senescent cell antigen-like-containing domain protein 1 isoform c
NCBI Official Synonym Full Names
LIM zinc finger domain containing 1
NCBI Official Symbol
LIMS1
NCBI Official Synonym Symbols
PINCH; PINCH1; PINCH-1
NCBI Protein Information
LIM and senescent cell antigen-like-containing domain protein 1
UniProt Protein Name
LIM and senescent cell antigen-like-containing domain protein 1
UniProt Gene Name
LIMS1
UniProt Synonym Gene Names
PINCH; PINCH1; PINCH-1
UniProt Entry Name
LIMS1_HUMAN

NCBI Description

The protein encoded by this gene is an adaptor protein which contains five LIM domains, or double zinc fingers. The protein is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that this protein may play a role in integrin-mediated cell adhesion or spreading. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Adapter protein in a cytoplasmic complex linking beta-integrins to the actin cytoskeleton, bridges the complex to cell surface receptor tyrosine kinases and growth factor receptors. Involved in the regulation of cell survival, cell proliferation and cell differentiation.

Research Articles on LIMS1

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Product Notes

The LIMS1 lims1 (Catalog #AAA7045748) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LIMS1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LIMS1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the LIMS1 lims1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIMS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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