Rabbit anti-Human DFF35, 45 Polyclonal Antibody | anti-DFFA antibody

DFF35, 45 (ICAD, DNA Fragmentation Factor, Inhibitor of Caspase Activated Deoxyribonuclease)

Cat. Num. AAA617288

• Gene Names: DFFA; DFF1; ICAD; DFF-45
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: DFF35; 45; Polyclonal Antibody; 45 (ICAD; DNA Fragmentation Factor; Inhibitor of Caspase Activated Deoxyribonuclease); Anti -DFF35; anti-DFFA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Detects endogenous levels of total full length DFF45 and/or full length DFF35. This antibody does not crossreact with related proteins.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

• Applicable Applications for anti-DFFA antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Dilution: Western Blot: 1:1000; Immunoprecipitation: 1:50
• Immunogen: Synthetic peptide (KLH coupled) corresponding to residues around Asp224 of human DFF45.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis of Jurkat cells, untreated (control) or etoposide-treated (25 uM), using DFF45/DFF35 Antibody.)

Related Product Information for anti-DFFA antibody

Human DFF45 and its mouse homologue ICAD function in normal cells as chaperones for caspase-activated deoxyribonuclease (DFF40 or CAD) during its synthesis (1). The association of DFF45 (or its isoform DFF35) with DFF40 inhibits the DNAse activity of the latter (1-4). In vitro, DFF45 has been shown to be the target of several caspases including caspase-3,-6,-7,-8 and granzyme B (3). In vivo, caspase-3 is believed to be the primary enzyme responsible for processing DFF45 and for release of its C-terminal fragment (3,5). The cleavage of DFF45 inactivates its inhibitory function on DFF40 and causes nuclear DNA degradation by DFF40, leading to cell death (6,7).

Product Categories/Family for anti-DFFA antibody

Antibodies; Abs to Apoptosis; Caspase

NCBI and Uniprot Product Information

• NCBI GI #: 4926918
• NCBI GeneID: 1676
• UniProt Accession #: O00273; Q5T6G5; Q96I97; Q9Y6C6; Q53HN4; Q5T6G6
• Molecular Weight: 36,522 Da
• NCBI Official Full Name: DNA fragmentation factor DFF35
• NCBI Official Synonym Full Names: DNA fragmentation factor, 45kDa, alpha polypeptide
• NCBI Official Symbol: DFFA
• NCBI Official Synonym Symbols: DFF1; ICAD; DFF-45
• NCBI Protein Information: DNA fragmentation factor subunit alpha; DFF45; inhibitor of CAD; OTTHUMP00000001903; OTTHUMP00000001904; DNA fragmentation factor 45 kDa subunit
• UniProt Protein Name: DNA fragmentation factor subunit alpha
• Protein Family: DNA fragmentation factor
• UniProt Gene Name: DFFA
• UniProt Synonym Gene Names: DFF1; DFF45
• UniProt Entry Name: DFFA_HUMAN

NCBI Description

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

DFFA: Inhibitor of the caspase-activated DNase (DFF40). Heterodimer of DFFA and DFFB. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase

Chromosomal Location of Human Ortholog: 1p36.3-p36.2

Cellular Component: nucleoplasm; nuclear chromatin; cytoplasm; lipid particle; nucleus; cytosol

Molecular Function: protein binding

Biological Process: apoptosis; positive regulation of apoptosis; DNA fragmentation during apoptosis; cell structure disassembly during apoptosis