Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using Phospho-LIMK1-Thr508 antibody.|Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-LIMK1-Thr508 antibody.|Immunofluorescence staining of methanol-fixed Hela cells using Phospho-LIMK1-Thr508 antibody.)
Rabbit LIMK1-T508 Polyclonal Antibody | anti-LIMK1-T508 antibody
Anti-Phospho-LIMK1-T508 Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purification
Synonyms
LIMK1-T508; Polyclonal Antibody; Anti-Phospho-LIMK1-T508 Antibody; anti-LIMK1-T508 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Form/Format
Liquid
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-LIMK1-T508 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:100
IF: 1:100-1:200
IHC: 1:50-1:100
IF: 1:100-1:200
Immunogen
A phospho specific peptide corresponding to residues surrounding T508 of human LIMK1
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C (regular) and-80 degree C (long term). Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using Phospho-LIMK1-Thr508 antibody.|Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-LIMK1-Thr508 antibody.|Immunofluorescence staining of methanol-fixed Hela cells using Phospho-LIMK1-Thr508 antibody.)
Western Blot (WB)
(Western blot analysis of extracts from Hela cells, using Phospho-LIMK1-Thr508 antibody.|Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-LIMK1-Thr508 antibody.|Immunofluorescence staining of methanol-fixed Hela cells using Phospho-LIMK1-Thr508 antibody.)
Related Product Information for anti-LIMK1-T508 antibody
There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
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Product Notes
The LIMK1-T508 (Catalog #AAA4153123) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Phospho-LIMK1-T508 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's LIMK1-T508 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:100 IF: 1:100-1:200. Researchers should empirically determine the suitability of the LIMK1-T508 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LIMK1-T508, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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