Rabbit anti-Human LIMK1 Polyclonal Antibody | anti-LIMK1 antibody

LIMK1, Phosphorylated (Thr508) (LIMK1, LIMK, LIM domain kinase 1) (PE)

Cat. Num. AAA6316162

• Gene Names: LIMK1; LIMK; LIMK-1
• Reactivity: Human
• Applications: Dot Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LIMK1; Polyclonal Antibody; Phosphorylated (Thr508) (LIMK1; LIMK; LIM domain kinase 1) (PE); LIM domain kinase 1; anti-LIMK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-LIMK1 antibody: FLISA, Dot Blot (DB)
• Application Notes: FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Phospho-LIMK1 (Thr508) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected surrounding Thr508 of human LIMK1.
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Dot Blot (DB)

(Dot blot analysis of anti-Phospho-LIMK1 (Thr508) antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

Related Product Information for anti-LIMK1 antibody

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. [provided by RefSeq].

Product Categories/Family for anti-LIMK1 antibody

Antibodies; Protein Kinases

References

Roder, C., et al. Childs Nerv Syst (2010). In press: Borensztajn, K., et al. Thromb. Res. 125 (6), E323-E328 (2010): Saxena, M., et al. J Cancer Res Ther 6(2):167-171(2010). Mishima, T., et al. Biochem. Biophys. Res. Commun. 392(4):577-581(2010). Jugessur, A., et al. PLoS ONE 5 (7), E11493 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 4505001
• NCBI GeneID: 3984
• NCBI Accession #: NP_002305.1
• NCBI GenBank Nucleotide #: NM_002314.3
• UniProt Accession #: P53667; O15283; Q15820; Q15821; Q75MU3; Q9Y5Q1; B7Z6I8; D3DXF4; D3DXF5
• Molecular Weight: 68,729 Da
• NCBI Official Full Name: LIM domain kinase 1 isoform 1
• NCBI Official Synonym Full Names: LIM domain kinase 1
• NCBI Official Symbol: LIMK1
• NCBI Official Synonym Symbols: LIMK; LIMK-1
• NCBI Protein Information: LIM domain kinase 1; LIM motif-containing protein kinase
• UniProt Protein Name: LIM domain kinase 1
• Protein Family: LIM domain kinase
• UniProt Gene Name: LIMK1
• UniProt Synonym Gene Names: LIMK; LIMK-1
• UniProt Entry Name: LIMK1_HUMAN

NCBI Description

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]

Uniprot Description

LIMK1: a TKL kinase of the LISK family which mediates Rho signaling to cytoskeleton. Contains two N-terminal LIM motifs. Phosphorylated and activated by PAK1 and ROCK, downstream effectors of Rho. May be involved in brain development. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin and induces actin cytoskeletal changes. The LIM domain interacts with the cytoplasmic domain of NRG1. Binds ROCK1. Interacts with slingshot 1. Overexpressed in prostate tumors and prostate and breast cancer cell lines; manipulation of activity correlates with invasiveness in breast and prostate cancer models. Located within the 7q11.2 amplicon associated with metastatic prostate cancer. Loss of one copy of LIMK1 is the likely cause of visuospatial cognition defects seen in small chromosomal deletions associated with dominant Williams-Beuren syndrome Three splice variant have been identified.

Protein type: Protein kinase, TKL; Kinase, protein; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; LISK family; LIMK subfamily

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: nucleoplasm; neuron projection; focal adhesion; cytoplasm; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; zinc ion binding; protein heterodimerization activity; heat shock protein binding; ATP binding; protein kinase activity

Biological Process: axon guidance; nervous system development; positive regulation of axon extension; positive regulation of stress fiber formation; negative regulation of ubiquitin-protein ligase activity; ephrin receptor signaling pathway; innate immune response; positive regulation of actin filament bundle formation; actin cytoskeleton organization and biogenesis; signal transduction; protein amino acid phosphorylation; Rho protein signal transduction

Disease: Williams-beuren Syndrome