Rabbit LIMK1 Polyclonal Antibody | anti-LIMK1 antibody

LIMK1 Antibody BIOTIN

Cat. Num. AAA5400469

• Gene Names: LIMK1; LIMK; LIMK-1
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot
• Synonyms: LIMK1; Polyclonal Antibody; LIMK1 Antibody BIOTIN; BIOTIN-Conjugated LIM domain kinase 1 Antibody; LIM domain kinase 1 antibody; LIM motif-containing protein kinase antibody; LIMK antibody; LIMK-1 antibody; anti-LIMK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Concentration: 0.55-0.75 ug/ul in antibody stabilization buffer (varies by lot)
• Sequence Length: 647

• Applicable Applications for anti-LIMK1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: DB: 1:10,000; ELISA: 1:10,000; WB: 1:500
• Immunogen: Synthetic peptide taken within amino acid region 550-647 on human LIM Kinase 1 protein.
• Conjugation: BIOTIN
• Molecular Function: ATP binding; protein kinase activity; protein serine/threonine kinase activity; zinc ion binding; heat shock protein binding
• Subcellular Location: Cytoplasm; Nucleus
• Preparation and Storage: Store at -20 degree C for long term storage.

Related Product Information for anti-LIMK1 antibody

Overview: Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways.

Expression: Highest expression in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

NCBI and Uniprot Product Information

• NCBI GI #: 4505001
• NCBI GeneID: 3984
• NCBI Accession #: NP_002305.1
• NCBI GenBank Nucleotide #: NM_002314.3
• UniProt Accession #: P53667; O15283; Q15820; Q15821; Q75MU3; Q9Y5Q1; B7Z6I8; D3DXF4; D3DXF5
• Molecular Weight: 68,729 Da
• NCBI Official Full Name: LIM domain kinase 1 isoform 1
• NCBI Official Synonym Full Names: LIM domain kinase 1
• NCBI Official Symbol: LIMK1
• NCBI Official Synonym Symbols: LIMK; LIMK-1
• NCBI Protein Information: LIM domain kinase 1
• UniProt Protein Name: LIM domain kinase 1
• Protein Family: LIM domain kinase
• UniProt Gene Name: LIMK1
• UniProt Synonym Gene Names: LIMK; LIMK-1

NCBI Description

There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is a serine/threonine kinase that regulates actin polymerization via phosphorylation and inactivation of the actin binding factor cofilin. This protein is ubiquitously expressed during development and plays a role in many cellular processes associated with cytoskeletal structure. This protein also stimulates axon growth and may play a role in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Feb 2011]

Uniprot Description

Serine/threonine-protein kinase that plays an essential role in the regulation of actin filament dynamics. Acts downstream of several Rho family GTPase signal transduction pathways. Activated by upstream kinases including ROCK1, PAK1 and PAK4, which phosphorylate LIMK1 on a threonine residue located in its activation loop. LIMK1 subsequently phosphorylates and inactivates the actin binding/depolymerizing factors cofilin-1/CFL1, cofilin-2/CFL2 and destrin/DSTN, thereby preventing the cleavage of filamentous actin (F-actin), and stabilizing the actin cytoskeleton. In this way LIMK1 regulates several actin-dependent biological processes including cell motility, cell cycle progression, and differentiation. Phosphorylates TPPP on serine residues, thereby promoting microtubule disassembly. Stimulates axonal outgrowth and may be involved in brain development. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. Required for atypical chemokine receptor ACKR2-induced phosphorylation of cofilin (CFL1).