Lipase, Hepatic (LIPC) Recombinant Protein | LIPC recombinant protein

Recombinant Lipase, Hepatic (LIPC)

Cat. Num. AAA2010684

• Gene Names: Lipc; HL; Hpl; AI256194
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Lipase; Hepatic (LIPC); Recombinant Lipase; LIPC recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-SR SKVHLIGYSL GAHVSGFAGSSMDGKNKIGR ITGLDPAGPM FEGTSPNERL SPDDANFVDA IHTFTREHMG LSVGIKQPIAHYDFYPNGGS FQPGCHFLEL YKHIAEHGLN AITQTIKCAH ERSVHLFIDS LQHSDLQSIGFQCSDMGSFS QGLCLSCKKG RCNTLGYDIR KDRSGKSKRL FLITRAQSPF KVYHYQFKIQFINQIEKPVE PTFTMSLLGT KEEIKRIPIT LGEGITSNKT YSFLITLDKD IGELI
• Sequence Length: 510

• Applicable Applications for LIPC recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Ser159~Ile415 (Accession # P27656) with two N-terminal Tags, His-tag and GST-tag
• Subcellular Location: Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 7.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 31982278
• NCBI GeneID: 15450
• NCBI Accession #: NP_032306.2
• NCBI GenBank Nucleotide #: NM_008280.2
• UniProt Accession #: P27656; Q8VC44
• Molecular Weight: 60.3kDa
• NCBI Official Full Name: hepatic triacylglycerol lipase
• NCBI Official Synonym Full Names: lipase, hepatic
• NCBI Official Symbol: Lipc
• NCBI Official Synonym Symbols: HL; Hpl; AI256194
• NCBI Protein Information: hepatic triacylglycerol lipase; hepatic lipase; lipase member C
• UniProt Protein Name: Hepatic triacylglycerol lipase
• Protein Family: Spore germination lipase
• UniProt Gene Name: Lipc
• UniProt Synonym Gene Names: Hpl; HL
• UniProt Entry Name: LIPC_MOUSE

Uniprot Description

LIPC: Hepatic lipase has the capacity to catalyze hydrolysis of phospholipids, mono-, di-, and triglycerides, and acyl-CoA thioesters. It is an important enzyme in HDL metabolism. Hepatic lipase binds heparin. Defects in LIPC are the cause of hepatic lipase deficiency (HL deficiency). A disorder characterized by elevated levels of beta-migrating very low density lipoproteins, and abnormally triglyceride-rich low and high density lipoproteins. Belongs to the AB hydrolase superfamily. Lipase family.

Protein type: Secreted, signal peptide; Secreted; Phospholipase; Lipid Metabolism - glycerolipid; EC 3.1.1.3

Cellular Component: extracellular space; microvillus; cell surface; late endosome; early endosome; extracellular region

Molecular Function: heparin binding; triacylglycerol lipase activity; hydrolase activity; apolipoprotein binding; acylglycerol lipase activity; heparan sulfate proteoglycan binding; protein binding; phospholipase A1 activity; lipase activity; transferase activity, transferring acyl groups; low-density lipoprotein binding; lipid binding; lysophospholipase activity

Biological Process: cholesterol metabolic process; neutral lipid catabolic process; cholesterol transport; glycerophospholipid catabolic process; fatty acid metabolic process; protein oligomerization; phosphatidylcholine metabolic process; cholesterol homeostasis; triacylglycerol metabolic process; phosphatidic acid metabolic process; phosphatidylserine metabolic process; heparan sulfate proteoglycan biosynthetic process; triacylglycerol catabolic process; lipid metabolic process; phosphatidylethanolamine metabolic process; lipid catabolic process; fatty acid biosynthetic process