Human COMP ELISA Kit | COMP elisa kit

Human COMP PicoKine ELISA Kit

Cat. Num. AAA176495

• Gene Names: COMP; MED; EDM1; EPD1; PSACH; THBS5
• Reactivity: Human
• Synonyms: COMP; Human COMP PicoKine ELISA Kit; Cartilage oligomeric matrix protein; cartilage oligomeric matrix protein; cartilage oligomeric matrix protein (pseudoachondroplasia; epiphyseal dysplasia 1; multiple); Cartilage oligomeric matrix protein precursor; COMP_HUMAN; EDM 1; EDM1; EPD 1; EPD1; Epiphyseal dysplasia 1; Epiphyseal dysplasia 1 multiple; Epiphyseal dysplasia multiple 1; MED; MGC13181; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1; Pseudoachondroplasia; THBS 5; THBS5; Thrombospondin 5; Thrombospondin-5; Thrombospondin5; TSP5; COMP elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant Human COL1A1
• Sequence Length: 757

• Samples: Cell Culture Supernates, Serum And Plasma (Heparin, Edta, Citrate)
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COMP elisa kit

Principle of the Assay: The Picokine Human COL1A1 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human COL1A1 with a 96-well strip plate that is pre-coated with antibody specific for COL1A1. The detection antibody is a biotinylated antibody specific for COL1A1. The capture antibody is a monoclonal antibody from mouse, the detection antibody is a biotinylated polyclonal antibody from goat. The kit contains recombinant Human COL1A1 with immunogen: Expression system for standard: CHO, Immunogen sequence: Q23-K277 + G1094-L1464. The kit is analytically validated with ready to use reagents. To measure Human COL1A1, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human COL1A1 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human COL1A1 in the sample!!Background/Introduction: Collagen, type I, alpha 1, also known as COL1A1, is a human gene that encodes the major component of type I collagen, the fibrillar collagen found in most connective tissues, including cartilage. This gene is mapped to 17q21.33. And this gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.

NCBI and Uniprot Product Information

• NCBI GI #: 209572601
• NCBI GeneID: 1311
• UniProt Accession #: P49747; O14592; Q16388; Q16389; Q2NL86; Q8N4T2; B4DKJ3
• Molecular Weight: 77,214 Da
• NCBI Official Full Name: Cartilage oligomeric matrix protein
• NCBI Official Synonym Full Names: cartilage oligomeric matrix protein
• NCBI Official Symbol: COMP
• NCBI Official Synonym Symbols: MED; EDM1; EPD1; PSACH; THBS5
• NCBI Protein Information: cartilage oligomeric matrix protein; TSP5; thrombospondin-5; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia 1, multiple); cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia 1, multiple)
• UniProt Protein Name: Cartilage oligomeric matrix protein
• Protein Family: Complexin
• UniProt Gene Name: COMP
• UniProt Synonym Gene Names: COMP; TSP5
• UniProt Entry Name: COMP_HUMAN

NCBI Description

The protein encoded by this gene is a noncollagenous extracellular matrix (ECM) protein. It consists of five identical glycoprotein subunits, each with EGF-like and calcium-binding (thrombospondin-like) domains. Oligomerization results from formation of a five-stranded coiled coil and disulfides. Binding to other ECM proteins such as collagen appears to depend on divalent cations. Mutations can cause the osteochondrodysplasias pseudochondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). [provided by RefSeq, Jul 2008]

Uniprot Description

COMP: May play a role in the structural integrity of cartilage via its interaction with other extracellular matrix proteins such as the collagens and fibronectin. Can mediate the interaction of chondrocytes with the cartilage extracellular matrix through interaction with cell surface integrin receptors. Could play a role in the pathogenesis of osteoarthritis. Potent suppressor of apoptosis in both primary chondrocytes and transformed cells. Suppresses apoptosis by blocking the activation of caspase-3 and by inducing the IAP family of survival proteins (BIRC3, BIRC2, BIRC5 and XIAP). Essential for maintaining a vascular smooth muscle cells (VSMCs) contractile/differentiated phenotype under physiological and pathological stimuli. Maintains this phenotype of VSMCs by interacting with ITGA7. Defects in COMP are the cause of multiple epiphyseal dysplasia type 1 (EDM1). EDM is a generalized skeletal dysplasia associated with significant morbidity. Joint pain, joint deformity, waddling gait, and short stature are the main clinical signs and symptoms. EDM is broadly categorized into the more severe Fairbank and the milder Ribbing types. Defects in COMP are the cause of pseudoachondroplasia (PSACH). PSAC is a dominantly inherited chondrodysplasia characterized by short stature and early-onset osteoarthrosis. PSACH is more severe than EDM1 and is recognized in early childhood. Belongs to the thrombospondin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 19p13.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: heparin binding; heparan sulfate proteoglycan binding; collagen binding; protein binding; protease binding; extracellular matrix structural constituent; calcium ion binding

Biological Process: limb development; organ morphogenesis; extracellular matrix organization and biogenesis; apoptosis; cell adhesion; skeletal development; negative regulation of apoptosis

Disease: Pseudoachondroplasia; Epiphyseal Dysplasia, Multiple, 1