Rabbit anti-Human LIME Polyclonal Antibody | anti-LIME1 antibody

LIME Antibody

Cat. Num. AAA9629256

• Gene Names: LIME1; LIME; LP8067; dJ583P15.4
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: LIME; Polyclonal Antibody; LIME Antibody; dJ583P15.4; FLJ20406; Lck interacting membrane protein; Lck interacting molecule; Lck interacting transmembrane adaptor 1; LIME 1; LIME1; LP8067; RP4 583P15.5; anti-LIME1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: LIME Antibody detects endogenous levels of total LIME.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-LIME1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Post Translational Modifications: Palmitoylation of Cys-28 and Cys-31 is required for raft targeting.Phosphorylated on tyrosines upon TCR activation and/or CD4 coreceptor stimulation, or upon BCR stimulation; which leads to the recruitment of SH2-containing proteins.
• Subunit Structure: When phosphorylated in response to BCR activation, interacts with LYN, PIK3R1, PLCG2, and GRB2 (By similarity). When phosphorylated in response to TCR stimulation and/or CD4 co-stimulation, interacts with LCK, CSK, FYN, PTPN11/SHP2, GRB2, PIK3R1 and GRAP2.
• Tissue Specificity: Expressed in peripheral blood lymphocytes, lymphoid tissues, and liver. Present in T-cells and plasma cells, and in various hematopoietic cell lines (at protein level).
• Function: Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T-cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules.
• Note: Present in lipid rafts. Recruited to the immunological synapse upon conjugation of T-cell with antigen-presenting cell.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-LIME1 antibody

Cell membrane; Single-pass type III membrane protein; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 157388906
• NCBI GeneID: 54923
• NCBI Accession #: NP_060276.2
• NCBI GenBank Nucleotide #: NM_017806.2
• UniProt Accession #: Q9H400; Q5JWJ2; Q6XYB3; Q9NX69; E1P5K5; E1P5K6
• Molecular Weight: 20,841 Da
• NCBI Official Full Name: lck-interacting transmembrane adapter 1
• NCBI Official Synonym Full Names: Lck interacting transmembrane adaptor 1
• NCBI Official Symbol: LIME1
• NCBI Official Synonym Symbols: LIME; LP8067; dJ583P15.4
• NCBI Protein Information: lck-interacting transmembrane adapter 1; lck-interacting membrane protein; lck-interacting molecule
• UniProt Protein Name: Lck-interacting transmembrane adapter 1
• Protein Family: Lck-interacting transmembrane adapter
• UniProt Gene Name: LIME1
• UniProt Synonym Gene Names: LIME; Lck-interacting membrane protein
• UniProt Entry Name: LIME1_HUMAN

NCBI Description

LIME1 is a raft-associated transmembrane adaptor phosphoprotein that is preferentially expressed in hemopoietic cells, particularly T cells (Brdickova et al., 2003 [PubMed 14610046]; Hur et al., 2003 [PubMed 14610044]).[supplied by OMIM, Mar 2008]

Uniprot Description

LIME1: Involved in BCR (B-cell antigen receptor)-mediated signaling in B-cells and TCR (T-cell antigen receptor)-mediated T- cell signaling in T-cells. In absence of TCR signaling, may be involved in CD4-mediated inhibition of T-cell activation. Couples activation of these receptors and their associated kinases with distal intracellular events such as calcium mobilization or MAPK activation through the recruitment of PLCG2, GRB2, GRAP2, and other signaling molecules. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold; Membrane protein, integral

Chromosomal Location of Human Ortholog: 20q13.3

Cellular Component: extracellular space; plasma membrane; integral to membrane

Biological Process: B cell receptor signaling pathway; T cell receptor signaling pathway