Typical Testing Data/Standard Curve (for reference only)
Mouse Activation-induced cytidine deaminase (AICDA) ELISA Kit | AICDA elisa kit
Mouse Activation-induced cytidine deaminase (AICDA) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: AICDA ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-AICDA antibody and an AICDA-HRP conjugate. The assay sample and buffer are incubated together with AICDA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AICDA concentration since AICDA from samples and AICDA-HRP conjugate compete for the anti-AICDA antibody binding site. Since the number of sites is limited, as more sites are occupied by AICDA from the sample, fewer sites are left to bind AICDA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AICDA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a RNA-editing deaminase that is a member of the cytidine deaminase family. The protein is involved in somatic hypermutation, gene conversion, and class-switch recombination of immunoglobulin genes. Defects in this gene are the cause of autosomal recessive hyper-IgM immunodeficiency syndrome type 2 (HIGM2). [provided by RefSeq, Feb 2009]
Uniprot Description
AID: Single-stranded DNA-specific cytidine deaminase. Involved in somatic hypermutation, gene conversion, and class- switch recombination in B-lymphocytes. Required for several crucial steps of B-cell terminal differentiation necessary for efficient antibody responses. May also play a role in the epigenetic regulation of gene expression by participating in DNA demethylation. Strongly expressed in lymph nodes and tonsils. Belongs to the cytidine and deoxycytidylate deaminase family.
Protein type: EC 3.5.4.38; Hydrolase
Chromosomal Location of Human Ortholog: 12p13
Cellular Component: cytoplasm; exosome (RNase complex); nucleus
Molecular Function: protein binding; zinc ion binding; ubiquitin protein ligase binding; cytidine deaminase activity
Biological Process: somatic diversification of immunoglobulins; B cell differentiation; somatic hypermutation of immunoglobulin genes; isotype switching; cytidine deamination; mRNA processing
Disease: Immunodeficiency With Hyper-igm, Type 2
Research Articles on AICDA
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Product Notes
The Mouse AICDA aicda (Catalog #AAA7209054) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7209054 ELISA Kit recognizes Mouse AICDA. It is sometimes possible for the material contained within the vial of "Activation-induced cytidine deaminase (AICDA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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