Rabbit LIMD1 Polyclonal Antibody | anti-LIMD1 antibody

Anti-LIMD1 Antibody

Cat. Num. AAA415976

• Reactivity: Human. Predicted: Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity purified
• Synonyms: LIMD1; Polyclonal Antibody; Anti-LIMD1 Antibody; anti-LIMD1 antibody

• Host: Rabbit
• Reactivity: Human. Predicted: Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 3004

• Applicable Applications for anti-LIMD1 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: ELISA titer: 1:20,000-1:80,000; Western blot analysis: 1:1,000-1:2,000; Immunohistochemistry: 1:100-1:500; Optimal dilutions/concentrations should be determined by the end user.
• Storage Buffer: PBS, pH 7.4 with 0.02% Sodium Azide
• Immunogen: Rabbit polyclonal LIMD1 (1) antibody was raised against a recombinated human LIMD1 protein 395-593aa (BC117236).
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Immunohistochemistry (IHC)

References

(1) Foxler,D.E., Bridge,K.S., James,V., et al. The LIMD1 protein bridges an association between the prolyl hydroxylases and VHL to repress HIF-1 activity. Nat. Cell Biol. 14 (2), 201-208 (2012)

NCBI and Uniprot Product Information

• NCBI GI #: 109658679
• NCBI GeneID: 8994
• Molecular Weight: 72,190 Da
• NCBI Official Full Name: Homo sapiens LIM domains containing 1, mRNA
• NCBI Official Synonym Full Names: LIM domains containing 1
• NCBI Official Symbol: LIMD1
• NCBI Protein Information: LIM domain-containing protein 1
• UniProt Protein Name: LIM domain-containing protein 1
• Protein Family: LIM domain-containing protein
• UniProt Gene Name: LIMD1
• UniProt Entry Name: LIMD1_HUMAN

Uniprot Description

LIMD1: Adapter or scaffold protein which participates in the assembly of numerous protein complexes and is involved in several cellular processes such as cell fate determination, cytoskeletal organization, repression of gene transcription, cell-cell adhesion, cell differentiation, proliferation and migration. Positively regulates microRNA (miRNA)-mediated gene silencing and is essential for P-body formation and integrity. Acts as a hypoxic regulator by bridging an association between the prolyl hydroxylases and VHL enabling efficient degradation of HIF1A. Acts as a transcriptional corepressor for SNAI1- and SNAI2/SLUG- dependent repression of E-cadherin transcription. Negatively regulates the Hippo signaling pathway and antagonizes phosphorylation of YAP1. Inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1- responsive elements. Regulates osteoblast development, function, differentiation and stress osteoclastogenesis. Enhances the ability of TRAF6 to activate adapter protein complex 1 (AP-1) and negatively regulates the canonical Wnt receptor signaling pathway in osteoblasts. May act as a tumor suppressor by inhibiting cell proliferation. Belongs to the zyxin/ajuba family.

Protein type: Tumor suppressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: focal adhesion; adherens junction; cytoplasm; nucleus

Molecular Function: protein binding; zinc ion binding; transcription corepressor activity

Biological Process: cell migration; transcription, DNA-dependent; multicellular organismal development; cytoskeleton organization and biogenesis; miRNA-mediated gene silencing; signal transduction; osteoblast development; regulation of cell shape; regulation of transcription, DNA-dependent; response to hypoxia; negative regulation of osteoblast differentiation; cytoplasmic mRNA processing body assembly; negative regulation of transcription, DNA-dependent; phosphorylation