Western Blot (WB)
(Anti-CD85i rabbit polyclonal antibody at 1:500 dilution.Lane A: MCF7 Membrane Lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDaObserved band size:53 kDa)
Rabbit anti-Human LILRA1/LIR-6/CD85i Polyclonal Antibody | anti-LILRA1 antibody
Anti-LILRA1/LIR-6/CD85i Antibody, Rabbit Polyclonal
IP: 1-4uL/mg of lysate
Western Blot (WB)
(Anti-CD85i rabbit polyclonal antibody at 1:500 dilution.Lane A: MCF7 Membrane Lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDaObserved band size:53 kDa)
Western Blot (WB)
(Anti-CD85i rabbit polyclonal antibody at 1:500 dilution.Lane A: MCF7 Membrane Lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:53 kDaObserved band size:53 kDa)
Immunoprecipitation (IP)
(CD85i was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-CD85i rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD85i rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 53 kDaObserved band size: 70 kDa)
Immunoprecipitation (IP)
(CD85i was immunoprecipitated using:Lane A:0.5 mg MCF-7 Whole Cell Lysate2 uL anti-CD85i rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-CD85i rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 53 kDaObserved band size: 70 kDa)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes an activating member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein is predominantly expressed in B cells, interacts with major histocompatibility complex class I ligands, and contributes to the regulation of immune responses. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, May 2013]
Uniprot Description
LILRA1: May act as receptor for class I MHC antigens. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 19q13.4
Cellular Component: integral to membrane; plasma membrane
Molecular Function: transmembrane receptor activity; antigen binding
Biological Process: regulation of immune response; cell surface receptor linked signal transduction; immune system process; defense response
Research Articles on LILRA1
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Product Notes
The LILRA1 lilra1 (Catalog #AAA8100414) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-LILRA1/LIR-6/CD85i Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LILRA1/LIR-6/CD85i can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). WB: 1:500-1:2000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the LILRA1 lilra1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LILRA1/LIR-6/CD85i, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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