Rabbit LHX3 Polyclonal Antibody | anti-LHX3 antibody

LHX3 Antibody

Cat. Num. AAA9608739

• Gene Names: LHX3; LIM3; CPHD3; M2-LHX3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: LHX3; Polyclonal Antibody; LHX3 Antibody; CPHD 3; CPHD3; DKFZp762A2013; LHX 3; LHX3_HUMAN; LIM 3; LIM homeobox 3; LIM homeobox gene 3; LIM homeobox protein 3; LIM/homeobox protein Lhx3; LIM/homeodomain protein LHX3; Lim3; M2 LHX3; mLim-3; mLIM3; P LIM; anti-LHX3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: LHX3 antibody detects endogenous levels of total LHX3
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 402

• Applicable Applications for anti-LHX3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human LHX3
• Subcellular Location: Nucleus.
• Predicted Cross Reactivity: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
• Similarity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%), Xenopus (90%)
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of LHX3 using HepG2 whole cell lysates)

Related Product Information for anti-LHX3 antibody

Function: Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1 (By similarity). Required for the establishment of the specialized cells of the pituitary gland and the nervous system. Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1.
Subunit Structure: Interacts with POU1F1 (PubMed:26612202). At neuronal promoters, interacts with LDB1, in motor neurons LDB1 is displaced by ISL1 and a ternary complex is formed in which ISL1 contacts both LHX3 and LDB1 (By similarity).
Similarity: The LIM domain specifically interacts with the Pit-1 POU domain and is required for synergistic interactions with Pit-1, but not for basal transcriptional activation events.

NCBI and Uniprot Product Information

• NCBI GI #: 7657303
• NCBI GeneID: 8022
• NCBI Accession #: NP_055379.1
• NCBI GenBank Nucleotide #: NM_014564.4
• UniProt Accession #: Q9UBR4; Q5TB39; Q5TB40; Q9NZB5; Q9P0I8; Q9P0I9
• Molecular Weight: Observed: 43 kDa; Predicted: 44 kDa
• NCBI Official Full Name: LIM/homeobox protein Lhx3 isoform b
• NCBI Official Synonym Full Names: LIM homeobox 3
• NCBI Official Symbol: LHX3
• NCBI Official Synonym Symbols: LIM3; CPHD3; M2-LHX3
• NCBI Protein Information: LIM/homeobox protein Lhx3
• UniProt Protein Name: LIM/homeobox protein Lhx3
• Protein Family: LIM/homeobox protein
• UniProt Gene Name: LHX3
• UniProt Synonym Gene Names: LIM homeobox protein 3

NCBI Description

This gene encodes a member of a large family of proteins which carry the LIM domain, a unique cysteine-rich zinc-binding domain. The encoded protein is a transcription factor that is required for pituitary development and motor neuron specification. Mutations in this gene cause combined pituitary hormone deficiency 3. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Uniprot Description

Acts as a transcriptional activator. Binds to and activates the promoter of the alpha-glycoprotein gene, and synergistically enhances transcription from the prolactin promoter in cooperation with POU1F1/Pit-1 (). Required for the establishment of the specialized cells of the pituitary gland and the nervous system. Involved in the development of interneurons and motor neurons in cooperation with LDB1 and ISL1.