Rabbit anti-Human LGMN Polyclonal Antibody | anti-LGMN antibody

LGMN, NT (LGMN, PRSC1, Legumain, Asparaginyl endopeptidase, Protease, cysteine 1) (Biotin)

Cat. Num. AAA6315879

• Gene Names: LGMN; AEP; LGMN1; PRSC1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: LGMN; Polyclonal Antibody; NT (LGMN; PRSC1; Legumain; Asparaginyl endopeptidase; Protease; cysteine 1) (Biotin); cysteine 1; anti-LGMN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human LGMN.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-LGMN antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 83-112 in the N-terminal region of human LGMN, UniProt Accession: #Q99538. Species sequence homology: monkey
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293 cell line lysates (35ug/lane) usingMBS647828. This demonstrates that MBS647828 detected the LGMN protein (arrow).)

Western Blot (WB)

(Western Blot analysis in human kidney lysate Lysates (20ug/lane) using 037755(1:1000). Secondary IgG (HRP) Goat Anti-Rabbit IgG, 1:10,000). Predicted band size: 49kD. Blocking/Dilution buffer: 5% NFDM/TBST. This demonstrates that MBS647828 detected the LGMN protein (arrow).)

Related Product Information for anti-LGMN antibody

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform.

Product Categories/Family for anti-LGMN antibody

Antibodies; Enzymes; Protease (Proteinase; Peptidase)

References

Clerin, V., et al. Atherosclerosis 201(1):53-66(2008). Liu, Z., et al. Mol. Cell 29(6):665-678(2008). Oh, J.H., et al. Mamm. Genome 16(12):942-954(2005). Dusso, A.S., et al. Am. J. Physiol. Renal Physiol. 289 (1), F8-F28 (2005): Murthy, R.V., et al. Clin. Cancer Res. 11(6):2293-2299(2005).

NCBI and Uniprot Product Information

• NCBI GI #: 56682962
• NCBI GeneID: 5641
• NCBI Accession #: NP_005597.3
• NCBI GenBank Nucleotide #: NM_005606.6
• UniProt Accession #: Q99538; O00123; Q86TV2; Q86TV3; Q9BTY1
• Molecular Weight: 42,008 Da
• NCBI Official Full Name: legumain preproprotein
• NCBI Official Synonym Full Names: legumain
• NCBI Official Symbol: LGMN
• NCBI Official Synonym Symbols: AEP; LGMN1; PRSC1
• NCBI Protein Information: legumain; cysteine protease 1; protease, cysteine 1; asparaginyl endopeptidase; protease, cysteine, 1 (legumain)
• UniProt Protein Name: Legumain
• UniProt Gene Name: LGMN
• UniProt Synonym Gene Names: PRSC1
• UniProt Entry Name: LGMN_HUMAN

NCBI Description

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]

Uniprot Description

LGMN: Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Belongs to the peptidase C13 family.

Protein type: EC 3.4.22.34; Protease

Chromosomal Location of Human Ortholog: 14q32.1

Cellular Component: lysosomal lumen; lysosome

Molecular Function: cysteine-type endopeptidase activity; peptidase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; negative regulation of neuron apoptosis; proteolysis; proteolysis involved in cellular protein catabolic process; receptor catabolic process; renal system process; toll-like receptor signaling pathway; vacuolar protein processing; vitamin D metabolic process