Mouse FHL2 Monoclonal Antibody | anti-FHL2 antibody

Anti-FHL2 Antibody

Cat. Num. AAA415090

• Gene Names: FHL2; DRAL; AAG11; FHL-2; SLIM3; SLIM-3
• Applications: ELISA, Western Blot
• Purity: Affinity purified
• Synonyms: FHL2; Monoclonal Antibody; Anti-FHL2 Antibody; Anti-FHL-2 Antibody; anti-FHL2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Anti-FHL-2 recognizes FHL-2 expression in Hella whole cell lysate.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 279

• Applicable Applications for anti-FHL2 antibody: ELISA (EIA), Western Blot (WB-Ce)
• Application Notes: WB: 1:500, analysis of FHL-2 expression in Hella whole cell lysate with Anti-FHL-2
• Raised In: Purified mouse monoclonal antibody generated using E.coli expressed recombinant protein FHL-2 as immunogen.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Immunogen: Recombinant protein corresponding to full length FHL-2
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Testing Data

References

None

NCBI and Uniprot Product Information

• NCBI GI #: 87159836
• NCBI GeneID: 2274
• NCBI Accession #: NP_001034581.1
• NCBI GenBank Nucleotide #: NM_001039492.2
• UniProt Accession #: Q14192; Q13229; Q13644; Q2I5I4; Q5TM15; Q9P294
• Molecular Weight: 15,890 Da
• NCBI Official Full Name: four and a half LIM domains protein 2
• NCBI Official Synonym Full Names: four and a half LIM domains 2
• NCBI Official Symbol: FHL2
• NCBI Official Synonym Symbols: DRAL; AAG11; FHL-2; SLIM3; SLIM-3
• NCBI Protein Information: four and a half LIM domains protein 2; LIM domain protein DRAL; aging-associated gene 11; down-regulated in rhabdomyosarcoma LIM protein; skeletal muscle LIM-protein 3
• UniProt Protein Name: Four and a half LIM domains protein 2
• Protein Family: Four and a half LIM domains protein
• UniProt Gene Name: FHL2
• UniProt Entry Name: FHL2_HUMAN

NCBI Description

This gene encodes a member of the four-and-a-half-LIM-only protein family. Family members contain two highly conserved, tandemly arranged, zinc finger domains with four highly conserved cysteines binding a zinc atom in each zinc finger. This protein is thought to have a role in the assembly of extracellular membranes. Also, this gene is down-regulated during transformation of normal myoblasts to rhabdomyosarcoma cells and the encoded protein may function as a link between presenilin-2 and an intracellular signaling pathway. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

FHL2: May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation.

Protein type: Motility/polarity/chemotaxis; Nuclear receptor co-regulator; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 2q12.2

Cellular Component: nucleoplasm; focal adhesion; M band; nucleus; Z disc; actin cytoskeleton

Molecular Function: identical protein binding; protein binding; androgen receptor binding; zinc ion binding; transcription coactivator activity; transcription factor binding

Biological Process: osteoblast differentiation; transcription, DNA-dependent; atrial cardiac muscle cell development; positive regulation of transcription, DNA-dependent; androgen receptor signaling pathway; ventricular cardiac muscle cell development; response to hormone stimulus; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; negative regulation of apoptosis