Human LGALS3BP ELISA Kit | LGALS3BP elisa kit

Human LGALS3BP ELISA Kit

Cat. Num. AAA8248374

• Gene Names: LGALS3BP; 90K; M2BP; gp90; CyCAP; BTBD17B; MAC-2-BP; TANGO10B
• Reactivity: Human
• Synonyms: LGALS3BP; Human LGALS3BP ELISA Kit; M2BP; Galectin-3-binding protein; Basement membrane autoantigen p105; Lectin galactoside-binding soluble 3-binding protein; Mac-2-binding protein; MAC2BP; Mac-2 BP; Tumor-associated antigen 90K; LGALS3BP elisa kit

• Reactivity: Human
• Specificity: The Human LGALS3BP ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human LGALS3BP proteins within the range of 62.5 pg/ml - 4000 pg/ml.
• Sequence Length: 585

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LGALS3BP elisa kit

Principle of the Assay: The Human LGALS3BP ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human LGALS3BP in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human LGALS3BP coated on a 96-well plate. Standards and samples are pipetted into the wells and LGALS3BP present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human LGALS3BP antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of LGALS3BP bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: Galectin-3-binding protein is a protein that in humans is encoded by the LGALS3BP gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Using fluorescence in-situ hybridization, the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds to galectin 1.

NCBI and Uniprot Product Information

• NCBI GI #: 5031863
• NCBI GeneID: 3959
• NCBI Accession #: NP_005558.1
• NCBI GenBank Nucleotide #: NM_005567.3
• UniProt Accession #: Q08380; Q7M4S0; Q9UCH8; Q9UCH9; Q9UCI0
• Molecular Weight: 65,331 Da
• NCBI Official Full Name: galectin-3-binding protein
• NCBI Official Synonym Full Names: galectin 3 binding protein
• NCBI Official Symbol: LGALS3BP
• NCBI Official Synonym Symbols: 90K; M2BP; gp90; CyCAP; BTBD17B; MAC-2-BP; TANGO10B
• NCBI Protein Information: galectin-3-binding protein
• UniProt Protein Name: Galectin-3-binding protein
• Protein Family: Galectin-3-binding protein
• UniProt Gene Name: LGALS3BP
• UniProt Synonym Gene Names: M2BP; MAC2BP; Mac-2 BP
• UniProt Entry Name: LG3BP_HUMAN

NCBI Description

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. LGALS3BP has been found elevated in the serum of patients with cancer and in those infected by the human immunodeficiency virus (HIV). It appears to be implicated in immune response associated with natural killer (NK) and lymphokine-activated killer (LAK) cell cytotoxicity. Using fluorescence in situ hybridization the full length 90K cDNA has been localized to chromosome 17q25. The native protein binds specifically to a human macrophage-associated lectin known as Mac-2 and also binds galectin 1. [provided by RefSeq, Jul 2008]

Uniprot Description

LGALS3BP: Promotes intergrin-mediated cell adhesion. May stimulate host defense against viruses and tumor cells.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q25

Cellular Component: extracellular matrix; extracellular region; extracellular space

Biological Process: cellular defense response; platelet degranulation; signal transduction