Rabbit anti-Human LFNG Polyclonal Antibody | anti-LFNG antibody

LFNG, ID (LFNG, Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe, O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase) (Azide free) (HRP)

Cat. Num. AAA6315760

• Gene Names: LFNG; SCDO3
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: LFNG; Polyclonal Antibody; ID (LFNG; Beta-1; 3-N-acetylglucosaminyltransferase lunatic fringe; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase) (Azide free) (HRP); O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; anti-LFNG antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-LFNG antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50-100; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: LFNG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93~122 amino acids from the Central region of human LFNG.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(LFNG Antibody (Center) western blot analysis in HL-60,K562,Hela cell line lysates (35ug/lane).This demonstrates the LFNG antibody detected the LFNG protein (arrow).)

Immunohistochemistry (IHC)

(LFNG Antibody (Center) IHC analysis in formalin fixed and paraffin embedded skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LFNG Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LFNG Antibody (Center) flow cytometric analysis of HL-60 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-LFNG antibody

Antibodies; Enzymes; Glycosyltransferase

References

Dunwoodie, S.L. Biochim. Biophys. Acta 1792(2):100-111(2009). Reedijk, M., et al. Int. J. Oncol. 33(6):1223-1229(2008). Sparrow, D.B., et al. Am. J. Hum. Genet. 78(1):28-37(2006). Cole, S.E., et al. Dev. Cell 3(1):75-84(2002). Moran, J.L., et al. Mamm. Genome 10(6):535-541(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 93140999
• NCBI GeneID: 3955
• NCBI Accession #: NP_001035257.1
• NCBI GenBank Nucleotide #: NM_001040167.1
• UniProt Accession #: Q8NES3; O00589; Q96C39; Q9UJW5; B3KTY6; B5MCR5
• Molecular Weight: 41,773 Da
• NCBI Official Full Name: beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform a preproprotein
• NCBI Official Synonym Full Names: LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
• NCBI Official Symbol: LFNG
• NCBI Official Synonym Symbols: SCDO3
• NCBI Protein Information: beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
• UniProt Protein Name: Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
• Protein Family: Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
• UniProt Gene Name: LFNG
• UniProt Entry Name: LFNG_HUMAN

NCBI Description

This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

LFNG: Glycosyltransferase that initiates the elongation of O- linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning. Defects in LFNG are the cause of spondylocostal dysostosis type 3 (SCDO3). An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life. Belongs to the glycosyltransferase 31 family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transferase; Cell cycle regulation; EC 2.4.1.222

Chromosomal Location of Human Ortholog: 7p22.2

Cellular Component: extracellular region; integral to Golgi membrane; vesicle

Molecular Function: metal ion binding; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase activity

Biological Process: compartment specification; organ morphogenesis; somitogenesis; Notch signaling pathway; positive regulation of protein binding; ovarian follicle development; regulation of Notch signaling pathway; metabolic process; female meiosis; regulation of somitogenesis; positive regulation of Notch signaling pathway

Disease: Spondylocostal Dysostosis 3, Autosomal Recessive