Integrin Alpha V Recombinant Protein | ITGaV recombinant protein

Recombinant Integrin Alpha V (ITGaV)

Cat. Num. AAA2029529

• Gene Names: ITGAV; CD51; MSK8; VNRA; VTNR
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Integrin Alpha V; Recombinant Integrin Alpha V (ITGaV); ITGaV recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; P ILNQFTPANI SRQAHILLDC GEDNVCKPKL EVSVDSDQKK IYIGDDNPLT LIVKAQNQGE GAYEAELIVS IPLQADFIGV VRNNEALARL SCAFKTENQT RQVVCDLGNP MKAGTQLLAG LRFSVHQQSE MDTSVKFDLQ IQSSNLFDKV SPVVSHKVDL AVLAAVEIRG VSSPDHVFLPIPNW
• Sequence Length: 1012

• Applicable Applications for ITGaV recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 24.0KDa
• Accurate Molecular Mass (KD): 24kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Pro560~Trp744
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 223468597
• NCBI GeneID: 3685
• NCBI Accession #: NP_001138472.1
• NCBI GenBank Nucleotide #: NM_001145000.2
• Molecular Weight: 111,132 Da
• NCBI Official Full Name: integrin alpha-V isoform 3
• NCBI Official Synonym Full Names: integrin, alpha V
• NCBI Official Symbol: ITGAV
• NCBI Official Synonym Symbols: CD51; MSK8; VNRA; VTNR
• NCBI Protein Information: integrin alpha-V
• UniProt Protein Name: Integrin alpha-V
• Protein Family: Integrin
• UniProt Gene Name: ITGAV
• UniProt Synonym Gene Names: MSK8; VNRA
• UniProt Entry Name: ITAV_HUMAN

NCBI Description

This gene encodes a protein that is a member of the integrin superfamily. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein undergoes post-translational cleavage to yield disulfide-linked heavy and light chains that combine with multiple integrin beta chains to form different integrins. This protein has been shown to heterodimerize with beta 1, beta 3, beta 5, beta 6, and beta 8; the heterodimer of alpha v and beta 3 is the Vitronectin receptor. This protein interacts with several extracellular matrix proteins to mediate cell adhesion and may play a role in cell migration. It is proposed that this protein may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants that encode different protein isoforms. Note that the integrin alpha 5 and integrin alpha V chains are produced by distinct genes. [provided by RefSeq, Jan 2015]

Uniprot Description

ITGAV: The alpha-V integrins are receptors for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and vWF. They recognize the sequence R-G-D in a wide array of ligands. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Belongs to the integrin alpha chain family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q31-q32

Cellular Component: filopodium membrane; focal adhesion; cell surface; membrane; integral to plasma membrane; microvillus membrane; plasma membrane; phagocytic vesicle; integrin complex; external side of plasma membrane

Molecular Function: voltage-gated calcium channel activity; viral receptor activity; protein binding; opsonin binding; insulin-like growth factor I binding; protein kinase C binding; protease binding; extracellular matrix binding; transforming growth factor beta binding; fibronectin binding; metal ion binding

Biological Process: negative regulation of lipid transport; axon guidance; extracellular matrix organization and biogenesis; entry of virus into host cell; positive regulation of cell adhesion; cell-matrix adhesion; negative chemotaxis; entry of symbiont into host cell by promotion of host phagocytosis; regulation of phagocytosis; antigen processing and presentation of peptide antigen via MHC class I; positive regulation of cell proliferation; antigen processing and presentation of exogenous peptide antigen via MHC class I; angiogenesis; cell growth; cell adhesion; integrin-mediated signaling pathway; cell migration; negative regulation of lipoprotein metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; cell-substrate adhesion; heterotypic cell-cell adhesion; negative regulation of low-density lipoprotein receptor biosynthetic process; positive regulation of osteoblast proliferation; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration; apoptotic cell clearance; positive regulation of cell migration