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Western Blot (WB) (Western blotAll lanes:  LFNGantibody at 4.43ug/mlLane 1 : Mouse spleen tissueLane 2 : K562 whole cell lysateLane 3 : HL60 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 42,29,40,36 kDaObserved band size: 42 kDa)

Rabbit anti-Human, Mouse LFNG Polyclonal Antibody | anti-LFNG antibody

LFNG Antibody

Gene Names
LFNG; SCDO3
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Antigen Affinity Purified
Synonyms
LFNG; Polyclonal Antibody; LFNG Antibody; Beta-1; 3-N-acetylglucosaminyltransferase lunatic fringe; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase; anti-LFNG antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Antigen Affinity Purified
Form/Format
Liquid
Sequence Length
379
Applicable Applications for anti-LFNG antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Species
Human
Immunogen
Recombinant human Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe protein (1-250AA)
Conjugate
Non-conjugated
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Santa Cruz Alternative
Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-324094
Preparation and Storage
Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes:  LFNGantibody at 4.43ug/mlLane 1 : Mouse spleen tissueLane 2 : K562 whole cell lysateLane 3 : HL60 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 42,29,40,36 kDaObserved band size: 42 kDa)

Western Blot (WB) (Western blotAll lanes:  LFNGantibody at 4.43ug/mlLane 1 : Mouse spleen tissueLane 2 : K562 whole cell lysateLane 3 : HL60 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 42,29,40,36 kDaObserved band size: 42 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7044890 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human testis tissue using MBS7044890 at dilution 1:100)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7044890 at dilution 1:100)

Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human adrenal gland tissue using MBS7044890 at dilution 1:100)
Related Product Information for anti-LFNG antibody
Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning (By similarity).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,266 Da
NCBI Official Full Name
beta-1,3-N-acetylglucosaminyltransferase lunatic fringe isoform a
NCBI Official Synonym Full Names
LFNG O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferase
NCBI Official Symbol
LFNG
NCBI Official Synonym Symbols
SCDO3
NCBI Protein Information
beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
UniProt Protein Name
Beta-1,3-N-acetylglucosaminyltransferase lunatic fringe
UniProt Gene Name
LFNG
UniProt Entry Name
LFNG_HUMAN

NCBI Description

This gene is a member of the fringe gene family which also includes radical and manic fringe genes. They all encode evolutionarily conserved glycosyltransferases that act in the Notch signaling pathway to define boundaries during embryonic development. While their genomic structure is distinct from other glycosyltransferases, fringe proteins have a fucose-specific beta-1,3-N-acetylglucosaminyltransferase activity that leads to elongation of O-linked fucose residues on Notch, which alters Notch signaling. This gene product is predicted to be a single-pass type II Golgi membrane protein but it may also be secreted and proteolytically processed like the related proteins in mouse and Drosophila (PMID: 9187150). Mutations in this gene have been associated with autosomal recessive spondylocostal dysostosis 3. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]

Uniprot Description

Glycosyltransferase that initiates the elongation of O-linked fucose residues attached to EGF-like repeats in the extracellular domain of Notch molecules. Decreases the binding of JAGGED1 to NOTCH2 but not that of DELTA1. Essential mediator of somite segmentation and patterning ().

Research Articles on LFNG

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Product Notes

The LFNG lfng (Catalog #AAA7044890) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LFNG Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LFNG can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the LFNG lfng for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LFNG, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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