Western Blot (WB)
(Western Blot: Sample: Human Lung lysate; Primary Ab: 2ug/ml Rabbit Anti-Human LRRK2 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Rabbit anti-Human Leucine Rich Repeat Kinase 2 (LRRK2) Polyclonal Antibody | anti-LRRK2 antibody
Polyclonal Antibody to Leucine Rich Repeat Kinase 2 (LRRK2)
Immunohistochemistry: 5-20 ug/mL;1:50-200
Immunocytochemistry: 5-20 ug/mL;1:50-200
Optimal working dilutions must be determined by end user
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western Blot: Sample: Human Lung lysate; Primary Ab: 2ug/ml Rabbit Anti-Human LRRK2 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Human Lung lysate; Primary Ab: 2ug/ml Rabbit Anti-Human LRRK2 Antibody;Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody;)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded liver tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the leucine-rich repeat kinase family and encodes a protein with an ankryin repeat region, a leucine-rich repeat (LRR) domain, a kinase domain, a DFG-like motif, a RAS domain, a GTPase domain, a MLK-like domain, and a WD40 domain. The protein is present largely in the cytoplasm but also associates with the mitochondrial outer membrane. Mutations in this gene have been associated with Parkinson disease-8. [provided by RefSeq, Jul 2008]
Uniprot Description
LRRK2: a large multidomain protein kinase with a TKL-type kinase domain, multiple protein-protein interaction domains and a mitochondrial Rho domain (MIRO). May play a role in the etiology of Parkinson disease. May also have GTPase activity. Positively regulates autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway. The process involves activation of nicotinic acid adenine dinucleotide phosphate (NAADP) receptors, increase in lysosomal pH, and calcium release from lysosomes. Interacts with PARK2, PRDX3 and TPCN2. Expressed throughout the adult brain, but at a lower level than in heart and liver. Expressed in the cerebellum, cerebral cortex, medulla, spinal cord occipital pole, frontal lobe, temporal lobe and putamen. Expression is particularly high in brain dopaminoceptive areas. Defects in LRRK2 are the cause of Parkinson disease type 8 (PARK8). A slowly progressive neurodegenerative disorder characterized by bradykinesia, rigidity, resting tremor, postural instability, neuronal loss in the substantia nigra, and the presence of neurofibrillary MAPT (tau)-positive and Lewy bodies in some patients.
Protein type: EC 2.7.11.1; Kinase, protein; LRRK family; Protein kinase, Ser/Thr (non-receptor); Protein kinase, TKL; TKL group
Chromosomal Location of Human Ortholog: 12q12
Cellular Component: amphisome; autolysosome; axon; cell junction; cell soma; cytoplasm; cytoplasmic vesicle; cytosol; dendrite; dendrite cytoplasm; endoplasmic reticulum; endosome; extracellular exosome; extracellular space; Golgi apparatus; Golgi-associated vesicle; growth cone; inclusion body; intracellular; intracellular membrane-bound organelle; intracellular ribonucleoprotein complex; lysosome; microvillus; mitochondrial inner membrane; mitochondrial matrix; mitochondrial membrane; mitochondrial outer membrane; mitochondrion; neuron projection; nucleus; perikaryon; plasma membrane; postsynapse; synaptic vesicle membrane; terminal bouton; trans-Golgi network; Wnt signalosome
Molecular Function: actin binding; ATP binding; beta-catenin destruction complex binding; clathrin binding; GTP binding; GTP-dependent protein kinase activity; GTPase activator activity; GTPase activity; identical protein binding; ion channel binding; kinase activity; MAP kinase kinase activity; microtubule binding; peroxidase inhibitor activity; protein binding; protein homodimerization activity; protein kinase A binding; protein kinase activity; protein serine/threonine kinase activity; receptor signaling complex scaffold activity; Rho GTPase binding; SNARE binding; syntaxin-1 binding; tubulin binding
Biological Process: activation of MAPK activity; activation of MAPKK activity; autophagy; calcium-mediated signaling; cellular protein localization; cellular response to manganese ion; cellular response to oxidative stress; cellular response to starvation; determination of adult lifespan; endocytosis; excitatory postsynaptic potential; Golgi organization and biogenesis; GTP metabolic process; intracellular distribution of mitochondria; lysosome organization and biogenesis; MAPK cascade; mitochondrion localization; mitochondrion organization; negative regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway; negative regulation of excitatory postsynaptic potential; negative regulation of macroautophagy; negative regulation of neuron death; negative regulation of protein binding; negative regulation of protein phosphorylation; negative regulation of protein processing; neuromuscular junction development; neuron death; neuron projection morphogenesis; olfactory bulb development; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; phosphorylation; positive regulation of autophagy; positive regulation of canonical Wnt signaling pathway; positive regulation of dopamine receptor signaling pathway; positive regulation of GTPase activity; positive regulation of MAP kinase activity; positive regulation of programmed cell death; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; positive regulation of protein binding; positive regulation of protein import into nucleus, translocation; positive regulation of protein phosphorylation; positive regulation of protein ubiquitination; protein amino acid phosphorylation; protein autophosphorylation; reactive oxygen species metabolic process; regulation of autophagy; regulation of branching morphogenesis of a nerve; regulation of CAMKK-AMPK signaling cascade; regulation of dopamine receptor signaling pathway; regulation of locomotion; regulation of membrane potential; regulation of mitochondrial depolarization; regulation of mitochondrial fission; regulation of neuroblast proliferation; regulation of neuron death; regulation of neuron maturation; regulation of protein kinase A signaling; regulation of retrograde transport, endosome to Golgi; regulation of synaptic transmission, glutamatergic; regulation of synaptic vesicle exocytosis; regulation of synaptic vesicle transport; response to oxidative stress; tangential migration from the subventricular zone to the olfactory bulb; Wnt receptor signaling pathway through beta-catenin; Wnt signalosome assembly
Disease: Parkinson Disease 8, Autosomal Dominant
Research Articles on LRRK2
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Product Notes
The LRRK2 lrrk2 (Catalog #AAA2005994) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Leucine Rich Repeat Kinase 2 (LRRK2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Leucine Rich Repeat Kinase 2 (LRRK2) can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2 ug/mL;1:500-2000 Immunohistochemistry: 5-20 ug/mL;1:50-200 Immunocytochemistry: 5-20 ug/mL;1:50-200 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the LRRK2 lrrk2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Leucine Rich Repeat Kinase 2 (LRRK2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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