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Western Blot (WB)
(Western Blot: Sample. Lane1: Mouse Sp2/0 Cells; Lane2: Mouse Liver Tissue; Lane3: Mouse Pancreas Tissue.)
Rabbit anti-Mouse Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) Polyclonal Antibody | anti-LRG1 antibody
Polyclonal Antibody to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)
Gene Names
Lrg1; Lrg; Lrhg; 1300008B03Rik; 2310031E04Rik
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Leucine Rich Alpha-2-Glycoprotein 1 (LRG1); Polyclonal Antibody; Polyclonal Antibody to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1); anti-LRG1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against LRG1. It has been selected for its ability to recognize LRG1 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-SANLSTLVL RENQLREVSA QWLQGLDALG HLDLAENQLS SLPSGLLASL GALHTLDLGY NLLESLPEGL LRGPRRLQRL HLEGNRLQRL EDSLLAPQPF LRVLFLNDNQ LVGVATGSFQ GLQHLDMLDL SNNSLSSTPP GLWAFLGRPT RDMQDGFDIS HNPWICDKNL ADLCRWLVAN RNKMFSQNDT RCAGPEAMKG QRLLDVAELG SL
MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF-SANLSTLVL RENQLREVSA QWLQGLDALG HLDLAENQLS SLPSGLLASL GALHTLDLGY NLLESLPEGL LRGPRRLQRL HLEGNRLQRL EDSLLAPQPF LRVLFLNDNQ LVGVATGSFQ GLQHLDMLDL SNNSLSSTPP GLWAFLGRPT RDMQDGFDIS HNPWICDKNL ADLCRWLVAN RNKMFSQNDT RCAGPEAMKG QRLLDVAELG SL
Sequence Length
347
Applicable Applications for anti-LRG1 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant LRG1 (Ser136~Leu342) expressed in E.coli.
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2060759
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample. Lane1: Mouse Sp2/0 Cells; Lane2: Mouse Liver Tissue; Lane3: Mouse Pancreas Tissue.)
Western Blot (WB)
(Western Blot: Sample. Lane1: Mouse Sp2/0 Cells; Lane2: Mouse Liver Tissue; Lane3: Mouse Pancreas Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Esophagus Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Mouse Esophagus Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
37,431 Da
NCBI Official Full Name
leucine-rich alpha-2-glycoprotein
NCBI Official Synonym Full Names
leucine-rich alpha-2-glycoprotein 1
NCBI Official Symbol
Lrg1
NCBI Official Synonym Symbols
Lrg; Lrhg; 1300008B03Rik; 2310031E04Rik
NCBI Protein Information
leucine-rich alpha-2-glycoprotein
UniProt Protein Name
Leucine-rich HEV glycoprotein
Protein Family
UniProt Gene Name
Lrg1
Uniprot Description
LRG1:
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 17 29.17 cM|17 D
Cellular Component: extracellular space; intracellular membrane-bound organelle
Molecular Function: transforming growth factor beta receptor binding
Biological Process: brown fat cell differentiation; positive regulation of angiogenesis; positive regulation of endothelial cell proliferation; positive regulation of transforming growth factor beta receptor signaling pathway
Research Articles on LRG1
Similar Products
Product Notes
The LRG1 lrg1 (Catalog #AAA2002921) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Leucine Rich Alpha-2-Glycoprotein 1 (LRG1) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the LRG1 lrg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -SANLSTLVL RENQLREVSA QWLQGLDALG HLDLAENQLS SLPSGLLASL GALHTLDLGY NLLESLPEGL LRGPRRLQRL HLEGNRLQRL EDSLLAPQPF LRVLFLNDNQ LVGVATGSFQ GLQHLDMLDL SNNSLSSTPP GLWAFLGRPT RDMQDGFDIS HNPWICDKNL ADLCRWLVAN RNKMFSQNDT RCAGPEAMKG QRLLDVAELG SL. It is sometimes possible for the material contained within the vial of "Leucine Rich Alpha-2-Glycoprotein 1 (LRG1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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