Western Blot (WB)
(Western blot analysis of extracts from P19 cells(heat shock treatment), using Phospho-LETM1 (Thr192) Antibody. The lane on the left was treated with blocking peptide.)
Rabbit anti-Human, Mouse LETM1 Polyclonal Antibody | anti-LETM1 antibody
Phospho-LETM1 (Thr192) Antibody
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
LETM1; Polyclonal Antibody; Phospho-LETM1 (Thr192) Antibody; LETM 1; LETM1 and EF hand domain containing protein 1; mitochondrial; LETM1 and EF-hand domain-containing protein 1; Letm1; LETM1_HUMAN; Leucine zipper EF hand containing transmembrane protein 1; Leucine zipper-EF-hand-containing transmembrane protein 1; Mdm38 homolog; anti-LETM1 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-LETM1 (Thr192) Antibody detects endogenous levels of LETM1 only when phosphorylated at Thr192.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-LETM1 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:500-1:2000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human LETM1 around the phosphorylation site of Thr192.
Conjugation
Unconjugated
Fragment
Fab fragment
Subunit Structure
Homohexamer (By similarity). Can form 2 complexes: a major (300kDa) and a minor complex (500-600kDa). Interacts with BCS1L.
Similarity
Belongs to the LETM1 family.
Subcellular Location
Mitochondrion inner membrane>Single-pass membrane protein.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of extracts from P19 cells(heat shock treatment), using Phospho-LETM1 (Thr192) Antibody. The lane on the left was treated with blocking peptide.)
Western Blot (WB)
(Western blot analysis of extracts from P19 cells(heat shock treatment), using Phospho-LETM1 (Thr192) Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-LETM1 antibody
Mitochondrial proton/calcium antiporter that mediates proton-dependent calcium efflux from mitochondrion. Crucial for the maintenance of mitochondrial tubular networks and for the assembly of the supercomplexes of the respiratory chain. Required for the maintenance of the tubular shape and cristae organization. In contrast to SLC8B1/NCLX, does not constitute the major factor for mitochondrial calcium extrusion.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,245 Da
NCBI Official Full Name
LETM1 and EF-hand domain-containing protein 1, mitochondrial
NCBI Official Synonym Full Names
leucine zipper-EF-hand containing transmembrane protein 1
NCBI Official Symbol
LETM1
NCBI Protein Information
LETM1 and EF-hand domain-containing protein 1, mitochondrial; Mdm38 homolog
UniProt Protein Name
LETM1 and EF-hand domain-containing protein 1, mitochondrial
Protein Family
UniProt Gene Name
LETM1
UniProt Entry Name
LETM1_HUMAN
Similar Products
Product Notes
The LETM1 letm1 (Catalog #AAA9612827) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-LETM1 (Thr192) Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LETM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:500-1:2000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the LETM1 letm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LETM1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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