Western Blot (WB)
(Western blot analysis of extracts of mouse pancreas, using LEP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Mouse LEP Polyclonal Antibody | anti-LEP antibody
LEP Polyclonal Antibody
Gene Names
LEP; OB; OBS; LEPD
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Functional Assay
Purity
Affinity purification
Synonyms
LEP; Polyclonal Antibody; LEP Polyclonal Antibody; OB; OBS; Leptin; Obese protein; Obesity factor;; anti-LEP antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Sequence Length
167
Applicable Applications for anti-LEP antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS)
Application Notes
Western Blot (WB): 1:200-1:500
Immunohistochemistry (IHC): 1:50-1:100
Immunofluorescence (IF): 1:20-1:50
Flow Cytometry (FC/FACS): 1:20-1:50
Immunohistochemistry (IHC): 1:50-1:100
Immunofluorescence (IF): 1:20-1:50
Flow Cytometry (FC/FACS): 1:20-1:50
Immunogen
A synthetic peptide of human LEP
Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Preparation and Storage
Store at -20 degree C or -80 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of mouse pancreas, using LEP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of mouse pancreas, using LEP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Related Product Information for anti-LEP antibody
Leptin is a 16 kDa adipocytederived hormone that relays the status of the body's energy reserves to the hypothalamus, resulting in the suppression of appetite and modulation of energy expenditure (1,2). Leptin functions as a pleiotropic cytokine with multiple roles in immune function and reproduction (14). There are at least five isoforms of the leptin receptor, but only OBRb is signaling competent (1,2). OBRb is expressed on a number of cells, including T cells, B cells, keratinocytes, and others (16). Leptin/OBRb interactions induce Jak2, PI3K, Erk1/2, and Stat3 activation (1,2). OBRb is often highly expressed in many tumor types, including breast, pancreatic, and Tumor Initiating Stem Cells (TISCS), providing a potential mechanistic link for the tumor promoting effects of obesity (2,7).
References
1. Faggioni, R. et al. (2001) FASEB J 15, 256571. 2. Park, J. et al. (2011) Endocr Rev 32, 55070. 3. Mancuso, P. et al. (2011) J Immunol 186, 108190. 4. Hausman, G.J. and Barb, C.R. (2010) Endocr Dev 19, 3144 5. Kanda, N. and Watanabe, S. (2008) Endocrinology 149, 518998. 6. Tanaka, M. et al. (2011) J Neurosci 31, 837380. 7. Feldman, D.E. et al. (2012) Proc Natl Acad Sci U S A 109, 82934.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19kDa
NCBI Official Full Name
leptin
NCBI Official Synonym Full Names
leptin
NCBI Official Symbol
LEP
NCBI Official Synonym Symbols
OB; OBS; LEPD
NCBI Protein Information
leptin; obese protein; obesity factor; obese, mouse, homolog of; leptin (murine obesity homolog); leptin (obesity homolog, mouse)
UniProt Protein Name
Leptin
Protein Family
UniProt Gene Name
LEP
UniProt Synonym Gene Names
OB; OBS
UniProt Entry Name
LEP_HUMAN
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Product Notes
The LEP lep (Catalog #AAA126282) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LEP Polyclonal Antibody reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's LEP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FC/FACS). Western Blot (WB): 1:200-1:500 Immunohistochemistry (IHC): 1:50-1:100 Immunofluorescence (IF): 1:20-1:50 Flow Cytometry (FC/FACS): 1:20-1:50. Researchers should empirically determine the suitability of the LEP lep for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LEP, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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