Human Osteopontin (OPN) CLIA Kit | OPN clia kit

Human Osteopontin (OPN) CLIA Kit

Cat. Num. AAA2708880

• Gene Names: SPP1; OPN; BNSP; BSPI; ETA-1
• Reactivity: Human
• Applications: Chemiluminescence Immunoassay (CLIA)
• Synonyms: Osteopontin (OPN); Human Osteopontin (OPN) CLIA Kit; Osteopontin; SPP1; BNSP; BSPI; ETA1; Secreted Phosphoprotein 1; Bone Sialoprotein I; Early T-Lymphocyte Activation 1; Nephropontin; Urinary stone protein; Uropontin; OPN clia kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Osteopontin (OPN).; No significant cross-reactivity or interference between Osteopontin (OPN) and analogues was observed.
• Sequence Length: 300

• Applicable Applications for OPN clia kit: Chemiluminescence Immunoassay (CLIA)
• Assay Type: Double-antibody Sandwich
• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 0.14-100ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.05ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Osteopontin (OPN) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Osteopontin (OPN) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Test Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Osteopontin (OPN). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Osteopontin (OPN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Osteopontin (OPN) level in the sample or standard.
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 4759166
• NCBI GeneID: 6696
• NCBI Accession #: NP_000573.1
• NCBI GenBank Nucleotide #: NP_000573.1
• UniProt Accession #: P10451
• NCBI Official Full Name: osteopontin isoform OPN-b
• NCBI Official Synonym Full Names: secreted phosphoprotein 1
• NCBI Official Symbol: SPP1
• NCBI Official Synonym Symbols: OPN; BNSP; BSPI; ETA-1
• NCBI Protein Information: osteopontin
• UniProt Protein Name: Osteopontin
• UniProt Gene Name: SPP1
• UniProt Synonym Gene Names: BNSP; OPN; SPP-1
• UniProt Entry Name: OSTP_HUMAN

NCBI Description

The protein encoded by this gene is involved in the attachment of osteoclasts to the mineralized bone matrix. The encoded protein is secreted and binds hydroxyapatite with high affinity. The osteoclast vitronectin receptor is found in the cell membrane and may be involved in the binding to this protein. This protein is also a cytokine that upregulates expression of interferon-gamma and interleukin-12. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

Uniprot Description

osteopontin: Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction. Belongs to the osteopontin family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 4q22.1

Cellular Component: extracellular space; cell projection; perinuclear region of cytoplasm; apical part of cell; extracellular region

Molecular Function: protein binding; extracellular matrix binding; cytokine activity

Biological Process: neutrophil chemotaxis; extracellular matrix organization and biogenesis; decidualization; osteoblast differentiation; extracellular matrix disassembly; response to vitamin D; biomineral formation; response to steroid hormone stimulus; negative regulation of collateral sprouting of intact axon in response to injury; positive regulation of bone resorption; inflammatory response; cell adhesion; embryo implantation