Rabbit anti-Human PAF1 Polyclonal Antibody | anti-PAF1 antibody

PAF1, CT (PAF1, PD2, RNA polymerase II-associated factor 1 homolog, Pancreatic differentiation protein 2) (Biotin)

Cat. Num. AAA6330463

• Gene Names: PAF1; PD2; F23149_1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PAF1; Polyclonal Antibody; CT (PAF1; PD2; RNA polymerase II-associated factor 1 homolog; Pancreatic differentiation protein 2) (Biotin); Pancreatic differentiation protein 2; anti-PAF1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 531

• Applicable Applications for anti-PAF1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: PAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 438-467 amino acids from the C-terminal region of human PAF1.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis using MBS647864 in Hela cell line lysates (35ug/lane).)

Western Blot (WB)

(Western blot analysis of PAF1 (arrow) usingMBS647864. 293 cell lysates (2ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAF1 gene.)

Related Product Information for anti-PAF1 antibody

PAF1, parafibromin (CDC73; MIM 607393), LEO1 (MIM 610507), and CTR9 (MIM 609366) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]). The PAF complex also has a role in histone monoubiquitination (Zhu et al., 2005 [PubMed 16307923]).[supplied by OMIM].

Product Categories/Family for anti-PAF1 antibody

Antibodies; Enzymes; Polymerase

References

Muntean, A.G., et al. Cancer Cell 17(6):609-621(2010). Kim, J., et al. Cell 140(4):491-503(2010). Moniaux, N., et al. PLoS ONE 4 (9), E7077 (2009): Olsen, J.V., et al. Cell 127(3):635-648(2006). Moniaux, N., et al. Oncogene 25(23):3247-3257(2006).

NCBI and Uniprot Product Information

• NCBI GI #: 42476169
• NCBI GeneID: 54623
• NCBI Accession #: NP_061961.2
• NCBI GenBank Nucleotide #: NP_061961.2
• UniProt Accession #: Q8N7H5
• NCBI Official Full Name: RNA polymerase II-associated factor 1 homolog isoform 1
• NCBI Official Synonym Full Names: PAF1 homolog, Paf1/RNA polymerase II complex component
• NCBI Official Symbol: PAF1
• NCBI Official Synonym Symbols: PD2; F23149_1
• NCBI Protein Information: RNA polymerase II-associated factor 1 homolog
• UniProt Protein Name: RNA polymerase II-associated factor 1 homolog
• Protein Family: Proteasome
• UniProt Gene Name: PAF1
• UniProt Synonym Gene Names: PD2; hPAF1
• UniProt Entry Name: PAF1_HUMAN

NCBI Description

This gene encodes a subunit of the polymerase associated factor (PAF1) complex. The PAF1 complex interacts with RNA polymerase II and plays a role in transcription elongation as well as histone modifications including ubiquitylation and methylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Feb 2012]