Mouse Legumain (LGMN) ELISA Kit | LGMN elisa kit

Mouse Legumain (LGMN) ELISA Kit

Cat. Num. AAA451710

• Gene Names: Lgmn; AEP; Prsc1; AI746452; AU022324
• Reactivity: Mouse
• Synonyms: Legumain (LGMN); Mouse Legumain (LGMN) ELISA Kit; AEP; LGMN1; PRSC1; Protease; Cysteine 1; Asparaginyl endopeptidase; LGMN elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of LGMN. No significant cross-reactivity or interference between LGMN and analogues was observed.
• Sequence Length: 435

• Samples: Mouse serum, plasma, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: <0.058ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level LGMN were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level LGMN were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for LGMN elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of LGMN in mouse serum, plasma, tissue homogenates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to LGMN. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to LGMN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain LGMN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of LGMN in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7242187
• NCBI GeneID: 19141
• NCBI Accession #: NP_035305.1
• NCBI GenBank Nucleotide #: NM_011175.3
• UniProt Accession #: O89017
• Molecular Weight: 49,373 Da
• NCBI Official Full Name: legumain preproprotein
• NCBI Official Synonym Full Names: legumain
• NCBI Official Symbol: Lgmn
• NCBI Official Synonym Symbols: AEP; Prsc1; AI746452; AU022324
• NCBI Protein Information: legumain
• UniProt Protein Name: Legumain
• UniProt Gene Name: Lgmn
• UniProt Synonym Gene Names: Prsc1

NCBI Description

This gene encodes a member of the cysteine peptidase family C13 that plays an important role in the endosome/lysosomal degradation system. The encoded inactive preproprotein undergoes autocatalytic removal of the C-terminal inhibitory propeptide to generate the active endopeptidase that cleaves protein substrates on the C-terminal side of asparagine residues. Mice lacking the encoded protein exhibit defects in the lysosomal processing of proteins resulting in their accumulation in the lysosomes, and develop symptoms resembling hemophagocytic lymphohistiocytosis. [provided by RefSeq, Aug 2016]

Uniprot Description

Has a strict specificity for hydrolysis of asparaginyl bonds. Can also cleave aspartyl bonds slowly, especially under acidic conditions. May be involved in the processing of proteins for MHC class II antigen presentation in the lysosomal/endosomal system. Required for normal lysosomal protein degradation in renal proximal tubules. Required for normal degradation of internalized EGFR. Plays a role in the regulation of cell proliferation via its role in EGFR degradation.