Goat ALMS1 Polyclonal Antibody | anti-ALMS1 antibody

ALMS1 (Alstrom syndrome 1, ALSS, KIAA0328)

Cat. Num. AAA620707

• Gene Names: ALMS1; ALSS; KIAA0328; DKFZp686A118; DKFZp686D1828
• Reactivity: Canine, Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: ALMS1; Polyclonal Antibody; ALMS1 (Alstrom syndrome 1; ALSS; KIAA0328); Anti -ALMS1 (Alstrom syndrome 1; anti-ALMS1 antibody

• Host: Goat
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Recognizes human ALMS1 at ~200kD and 75kD in lysates of human T cell line Jurkat after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 461kD according to NP_055935.3. Species Crossreactivity: Canine, mouse and rat.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-ALMS1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: ELISA: 1:32000; Western Blot: 0.3ug/ml. Preliminary experiments gave bands at approx 200kD and 75kD in lysates of Human T cell line Jurkat after antibody staining. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands).
• Immunogen: Synthetic peptide of human ALMS1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of ALMS1 in paraffin embedded Human Testis using ALMS1 antibody (2ug/ml).)

Product Categories/Family for anti-ALMS1 antibody

Antibodies; Abs to Disease Markers

NCBI and Uniprot Product Information

• NCBI GI #: 110349786
• NCBI GeneID: 7840
• NCBI Accession #: NP_055935.4
• NCBI GenBank Nucleotide #: NM_015120.4
• UniProt Accession #: Q8TCU4; Q53S05; Q580Q8; Q86VP9; Q9Y4G4
• Molecular Weight: 460,965 Da
• NCBI Official Full Name: Alstrom syndrome protein 1
• NCBI Official Synonym Full Names: Alstrom syndrome 1
• NCBI Official Symbol: ALMS1
• NCBI Official Synonym Symbols: ALSS; KIAA0328; DKFZp686A118; DKFZp686D1828
• NCBI Protein Information: Alstrom syndrome protein 1; OTTHUMP00000202344; OTTHUMP00000202401
• UniProt Protein Name: Alstrom syndrome protein 1
• Protein Family: Alstrom syndrome protein
• UniProt Gene Name: ALMS1
• UniProt Synonym Gene Names: KIAA0328
• UniProt Entry Name: ALMS1_HUMAN

NCBI Description

This gene encodes a protein containing a large tandem-repeat domain. The mouse ortholog of this gene has been shown to function in ciliogenesis in inner medullary collecting duct cells. Mutations in this gene have been associated with Alstrom syndrome. Alternative splice variants have been described but their full length sequences have not been determined.

Uniprot Description

ALMS1: Involved in PCM1-dependent intracellular transport. Required, directly or indirectly, for the localization of NCAPD2 to the proximal ends of centrioles. Required for proper formation and/or maintenance of primary cilia (PC), microtubule-based structures that protrude from the surface of epithelial cells. Defects in ALMS1 are the cause of Alstrom syndrome (ALMS). Alstrom syndrome is a rare autosomal recessive disorder characterized by progressive cone-rod retinal dystrophy, neurosensory hearing loss, early childhood obesity and type 2 diabetes mellitus. Dilated cardiomyopathy, acanthosis nigricans, male hypogonadism, hypothyroidism, developmental delay and hepatic dysfunction can also be associated with the syndrome. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: nucleoplasm; centriole; spindle pole; centrosome; cytoplasm; cytosol; nucleus; cilium

Molecular Function: protein binding

Biological Process: regulation of stress fiber formation; organelle organization and biogenesis; mitotic cell cycle; endosome transport; G2/M transition of mitotic cell cycle

Disease: Alstrom Syndrome