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Western Blot (WB) (FancD2 antibody (pAb) tested by Immunoprecipitation. 10 ul of FancD2 antibody was used to immunoprecipitate FancD2 from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the FancD2 antibody at a dilution of 1:500.)

Rabbit anti-Human FancD2 Polyclonal Antibody | anti-FancD2 antibody

FancD2 antibody (pAb)

Reactivity
Human
Applications
Immunoprecipitation, Western Blot
Purity
Affinity Purified
Synonyms
FancD2; Polyclonal Antibody; FancD2 antibody (pAb); FA4; FAD; FACD; FAD2; FA-D2; FANCD; anti-FancD2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purified
Form/Format
Purified IgG in 70mM Tris (pH8), 105mM NaCl, 31mM glycine, 0.07mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Applicable Applications for anti-FancD2 antibody
Immunoprecipitation (IP), Western Blot (WB)
Application Notes
Applications Validated:
IP: 10ul per IP
WB: 1:500-1:5,000 dilution
Immunogen
This FancD2 antibody was raised against a peptide within the internal region of human FancD2.
Preparation and Storage
Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20 degree C for up to 2 years. Keep all reagents on ice when not in storage.1ug/ul

Western Blot (WB)

(FancD2 antibody (pAb) tested by Immunoprecipitation. 10 ul of FancD2 antibody was used to immunoprecipitate FancD2 from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the FancD2 antibody at a dilution of 1:500.)

Western Blot (WB) (FancD2 antibody (pAb) tested by Immunoprecipitation. 10 ul of FancD2 antibody was used to immunoprecipitate FancD2 from 400 ug of HeLa whole cell extract (lane 2). 10 ul of rabbit IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the FancD2 antibody at a dilution of 1:500.)

Western Blot (WB)

(Western blot of FancD2 antibody. Lane 1: HeLa whole-cell extract (40 ug). Lane 2: Nuclear extract (40 ug) of K-562 cells. Both probed with FancD2 antibody (pAb) at a 1:500 dilution.)

Western Blot (WB) (Western blot of FancD2 antibody. Lane 1: HeLa whole-cell extract (40 ug). Lane 2: Nuclear extract (40 ug) of K-562 cells. Both probed with FancD2 antibody (pAb) at a 1:500 dilution.)
Related Product Information for anti-FancD2 antibody
Short Description: Our FancD2 antibody (pAb) was raised in a Rabbit host. It has been validated for use in Immunoprecipitation and Western blot; it has been shown to react with Human samples.

Background: FancD2 (Fanconi anemia complementation group D2) is a protein encoded by a gene mutated in Fanconi anemia, a recessive genetic disorder characterized by chromosomal instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. FancD2 is monoubiquitinated in response to DNA damage and is required for maintenance of chromosomal stability and the repair of DNA double-strand breaks. FancD2 also likely plays a role in activating the S-phase and G2/M DNA damage checkpoints. It promotes the loading of BRCA2 and FANCD1 onto damaged chromatin and may also be involved in B-cell immunoglobulin isotype switching.
Product Categories/Family for anti-FancD2 antibody

NCBI and Uniprot Product Information

Molecular Weight
150kDa

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Product Notes

The FancD2 (Catalog #AAA388739) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FancD2 antibody (pAb) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FancD2 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP), Western Blot (WB). Applications Validated: IP: 10ul per IP WB: 1:500-1:5,000 dilution. Researchers should empirically determine the suitability of the FancD2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FancD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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