Sheep anti-Human LEF-1 Polyclonal Antibody | anti-LEF-1 antibody

LEF-1

Cat. Num. AAA395904

• Gene Names: LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA
• Reactivity: Human
• Applications: Western Blot
• Purity: Ammonium Sulfate Precipitation
• Synonyms: LEF-1; Polyclonal Antibody; lef-1; sheep anti human lymphoid enhancer factor-1; anti-LEF-1 antibody

• Host: Sheep
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Ammonium Sulfate Precipitation
• Form/Format: Provided as solution in phosphate buffered saline with 0.08% sodium azide
• Sequence Length: 371

• Applicable Applications for anti-LEF-1 antibody: Western Blot (WB)
• Immunogen: A full length protein
• Preparation and Storage: Product should be stored at -20 degree C. Aliquot to avoid freeze/thaw cycles

Related Product Information for anti-LEF-1 antibody

The immunogen for the anti-LEF-1 antibody was a full length protein. Lymphoid Enhancer Factor -1 is a transcription factor of the High Mobility Group of DNA binding proteins. It is one member of a family of four proteins referred to as LEF/TCF transcription factors (LEF-1, TCF-1, TCF-3 and TCF-4). These factors play a crucial role in WNT/WIngless signaling, a signal transduction cascade that directs cell differentiation. Aberrant activation of the WNT/Wingless pathway is also a root cause in the genesis of certain cancers such as colon cancer, melanoma and breast cancer.LEF-1 is expressed during development in many different differentiating tissues, and in a few tissues after birth. LEF-1 expression is required for proper development of breast, teeth, hair, whiskers and the trigeminal nerve. It is redundant with TCF-1 (for T Cell Factor-1) for correct development of T lymphocytes in the thymus. LEF-1 is a 399 amino acid protein that appears as a cluster of [polypeptides ranging in size between 55-57 kD on SDS-PAGE analysis. Alternative splice forms of LEF-1 and other LEF/TCF family members have been identified, but the expression pattern and relative abundance of these alternative forms have not been well studied. They can appear on westerns as smaller immunoreactive forms.

Product Categories/Family for anti-LEF-1 antibody

Polyclonal Antibody; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 195222732
• NCBI GeneID: 51176
• NCBI Accession #: NP_001124185.1
• NCBI GenBank Nucleotide #: NM_001130713.2
• UniProt Accession #: Q9UJU2; Q3ZCU4; Q9HAZ0; B4DG38; B7Z8E2; E9PDK3
• Molecular Weight: 44.2
• NCBI Official Full Name: lymphoid enhancer-binding factor 1 isoform 2
• NCBI Official Synonym Full Names: lymphoid enhancer-binding factor 1
• NCBI Official Symbol: LEF1
• NCBI Official Synonym Symbols: LEF-1; TCF10; TCF7L3; TCF1ALPHA
• NCBI Protein Information: lymphoid enhancer-binding factor 1; TCF1-alpha; T cell-specific transcription factor 1-alpha
• UniProt Protein Name: Lymphoid enhancer-binding factor 1
• Protein Family: Late expression factor
• UniProt Gene Name: LEF1
• UniProt Synonym Gene Names: LEF-1; TCF1-alpha
• UniProt Entry Name: LEF1_HUMAN

NCBI Description

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

Function: Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. May play a role in hair cell differentiation and follicle morphogenesis. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1

By similarity. Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling. Isoform 5 transcriptionally activates the fibronectin promoter, binds to and represses transcription from the E-cadherin promoter in a CTNNB1-independent manner, and is involved in reducing cellular aggregation and increasing cell migration of pancreatic cancer cells. Isoform 1 transcriptionally activates MYC and CCND1 expression and enhances proliferation of pancreatic tumor cells. Ref.1 Ref.3 Ref.13

Subunit structure: Binds the armadillo repeat of CTNNB1 and forms a stable complex. Interacts with EP300, TLE1 and PIASG

By similarity. Binds ALYREF/THOC4, MDFI and MDFIC. Interacts with NLK. Ref.9 Ref.10 Ref.11 Ref.13 Ref.14 Ref.15

Subcellular location: Nucleus

By similarity. Note: Found in nuclear bodies upon PIASG binding

By similarity.

Tissue specificity: Detected in thymus. Not detected in normal colon, but highly expressed in colon cancer biopsies and colon cancer cell lines. Expressed in several pancreatic tumors and weakly expressed in normal pancreatic tissue. Isoforms 1 and 5 are detected in several pancreatic cell lines. Ref.3

Domain: Proline-rich and acidic regions are implicated in the activation functions of RNA polymerase II transcription factors.

Post-translational modification: Phosphorylated at Thr-155 and/or Ser-166 by NLK. Phosphorylation by NLK at these sites represses LEF1-mediated transcriptional activation of target genes of the canonical Wnt signaling pathway. Ref.15

Sequence similarities: Belongs to the TCF/LEF family.Contains 1 HMG box DNA-binding domain.