Chicken LDL-R Polyclonal Antibody | anti-LDLR antibody

LDL-R Antibody

Cat. Num. AAA150298

• Gene Names: LDLR; FH; FHC; LDLCQ2
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: LDL-R Antibody is affinity chromatography purified via peptide column.
• Synonyms: LDL-R; Polyclonal Antibody; LDL-R Antibody; FH; FHC; LDLCQ2; Low-density lipoprotein receptor; LDL receptor; low density lipoprotein receptor; anti-LDLR antibody

• Host: Chicken
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgY
• Purity/Purification: LDL-R Antibody is affinity chromatography purified via peptide column.
• Form/Format: Liquid
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 860

• Applicable Applications for anti-LDLR antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: LDL-R antibody can be used for detection of LDL-R by Western blot at 1 - 2 mug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 mug/mL. For immunofluorescence start at 20 mug/mL.
• Conjugate: Unconjugated
• Immunogen: LDL-R antibody was raised against an 18 amino acid synthetic peptide near the center of human LDL-R.
• Buffer: LDL-R Antibody is supplied in PBS containing 0.02% sodium azide.
• Preparation and Storage: LDL-R antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of LDL-R in human liver tissue lysate with LDL-R antibody at (A) 1 and (B) 2 μg/mL.)

Immunohistochemistry (IHC)

(Immunohistochemistry of LDL-R in human liver tissue with LDL-R antibody at 2.5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of LDLR in human liver tissue with LDLR antibody at 20 μg/mL.)

Related Product Information for anti-LDLR antibody

LDL-R Antibody: The low density lipoprotein receptor (LDL-R) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in the LDL-R gene cause the autosomal dominant disorder, familial hypercholesterolemia. Along with SCARB1, CLDN1, and the tetraspanin superfamily member CD81, LDL-R has been reported to be an entry factor for the Hepatitis C virus. At least three isoforms of LDL-R are known to exist.

NCBI and Uniprot Product Information

• NCBI GI #: 4504975
• NCBI GeneID: 3949
• NCBI Accession #: NP_000518
• NCBI GenBank Nucleotide #: NM_000527.4
• UniProt Accession #: P01130; Q53ZD9; Q59FQ1; Q9UDH7; B4DII3; B4DJZ8; B4DR00; B4DTQ3; C0JYY8; H0YLU8; H0YNT7
• Molecular Weight: 82,255 Da
• NCBI Official Full Name: low-density lipoprotein receptor isoform 1
• NCBI Official Synonym Full Names: low density lipoprotein receptor
• NCBI Official Symbol: LDLR
• NCBI Official Synonym Symbols: FH; FHC; LDLCQ2
• NCBI Protein Information: low-density lipoprotein receptor; LDL receptor; low-density lipoprotein receptor class A domain-containing protein 3
• UniProt Protein Name: Low-density lipoprotein receptor
• Protein Family: Low-density lipoprotein receptor
• UniProt Gene Name: LDLR
• UniProt Synonym Gene Names: LDL receptor
• UniProt Entry Name: LDLR_HUMAN

NCBI Description

The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]

Uniprot Description

LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: Golgi apparatus; cell surface; membrane; integral to plasma membrane; lysosome; early endosome; late endosome; plasma membrane; coated pit; endosome membrane; receptor complex; external side of plasma membrane

Molecular Function: very-low-density lipoprotein receptor activity; low-density lipoprotein receptor activity; protein binding; low-density lipoprotein binding; calcium ion binding; clathrin heavy chain binding; glycoprotein binding

Biological Process: lipoprotein catabolic process; phototransduction, visible light; receptor-mediated endocytosis; cholesterol metabolic process; cholesterol transport; viral reproduction; cholesterol absorption; endocytosis; lipoprotein metabolic process; cholesterol homeostasis; phospholipid transport; lipid metabolic process; retinoid metabolic process

Disease: Hypercholesterolemia, Familial