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Western Blot (WB) (LDHD Antibody (N-term) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the LDHD antibody detected the LDHD protein (arrow).)

Rabbit anti-Human LDHD Polyclonal Antibody | anti-LDHD antibody

LDHD Antibody (N-term)

Gene Names
LDHD; DLD
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
LDHD; Polyclonal Antibody; LDHD Antibody (N-term); Probable D-lactate dehydrogenase; mitochondrial; DLD; Lactate dehydrogenase D; LDHD HGNC:19708); anti-LDHD antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This LDHD antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 35-62 amino acids from the N-terminal region of human LDHD.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
35-62
Sequence Length
507
Applicable Applications for anti-LDHD antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(LDHD Antibody (N-term) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the LDHD antibody detected the LDHD protein (arrow).)

Western Blot (WB) (LDHD Antibody (N-term) western blot analysis in HepG2 cell line lysates (15ug/lane).This demonstrates the LDHD antibody detected the LDHD protein (arrow).)

Immunohistochemistry (IHC)

(LDHD Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LDHD Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (LDHD Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the LDHD Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(LDHD Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (LDHD Antibody (N-term) flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-LDHD antibody
LDHD belongs to the D-isomer
specific 2-hydroxyacid dehydrogenase family. The similar protein in
yeast has both D-lactate and D-glycerate dehydrogenase activities.
Alternative splicing occurs at this locus and two transcript
variants encoding distinct isoforms have been identified. [provided
by RefSeq].
Product Categories/Family for anti-LDHD antibody
References
Dastani, Z., et al. Eur. J. Hum. Genet. (2009) In press :
Flick, M.J., et al. Biochem. Biophys. Res. Commun. 295(4):910-916(2002)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54871
NCBI Official Full Name
probable D-lactate dehydrogenase, mitochondrial isoform 1
NCBI Official Synonym Full Names
lactate dehydrogenase D
NCBI Official Symbol
LDHD
NCBI Official Synonym Symbols
DLD
NCBI Protein Information
probable D-lactate dehydrogenase, mitochondrial
UniProt Protein Name
Probable D-lactate dehydrogenase, mitochondrial
UniProt Gene Name
LDHD
UniProt Synonym Gene Names
DLD; Lactate dehydrogenase D
UniProt Entry Name
LDHD_HUMAN

NCBI Description

The protein encoded by this gene belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The similar protein in yeast has both D-lactate and D-glycerate dehydrogenase activities. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

LDH-D: belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. The similar protein in yeast has both D-lactate and D-glycerate dehydrogenase activities. Alternative splicing occurs at this locus and two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Protein type: Carbohydrate Metabolism - pyruvate; Mitochondrial; Oxidoreductase; EC 1.1.2.4

Chromosomal Location of Human Ortholog: 16q23.1

Cellular Component: mitochondrion; mitochondrial inner membrane

Molecular Function: UDP-N-acetylmuramate dehydrogenase activity; protein binding; FAD binding; D-lactate dehydrogenase (cytochrome) activity

Research Articles on LDHD

Similar Products

Product Notes

The LDHD ldhd (Catalog #AAA9201925) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 35-62. The LDHD Antibody (N-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's LDHD can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the LDHD ldhd for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "LDHD, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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