Rabbit LAT Polyclonal Antibody | anti-LAT antibody

Phospho-LAT (Tyr255) Antibody

Cat. Num. AAA9614437

• Gene Names: LAT; LAT1; pp36
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (88%), Bovine (88%), Horse (100%), Sheep (88%), Rabbit (88%)
• Applications: Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
• Purity: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Synonyms: LAT; Polyclonal Antibody; Phospho-LAT (Tyr255) Antibody; 36kDa phospho tyrosine adapter protein; 36kDa phospho-tyrosine adapter protein; 36kDa phospho-tyrosine adaptor protein; LAT 1; lat; LAT_HUMAN; LAT1; Linker for activation of T cells; Linker for activation of T cells family member 1; linker for activation of T cells; transmembrane adaptor; Linker for activation of T-cells family member 1; p36 38; p36-38; pp36; anti-LAT antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig (88%), Bovine (88%), Horse (100%), Sheep (88%), Rabbit (88%)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Phospho-LAT (Tyr255) Antibody detects endogenous levels of LAT only when phosphorylated at Tyr255.; Tissue Specificity: Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level).
• Purity/Purification: The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-LAT antibody: Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), Peptide ELISA (EIA)
• Application Notes: WB: 1:1000-3000; IF/ICC: 1:100-1:500; Peptide ELISA: 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human LAT around the phosphorylation site of Tyr255.
• Conjugation: Unconjugated
• Fragment: Fab fragment
• Post Translational Modifications: Phosphorylated on tyrosines by ZAP70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ. Phosphorylated by ITK leading to the recruitment of VAV1 to LAT-containing complexes.Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation.
• Subunit Structure: When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB, SKAP2 and CLNK (By similarity). Interacts with FCGR1A. Interacts with GRB2, PLCG1 and THEMIS upon TCR activation in thymocytes (By similarity).
• Subcellular Location: Cell membrane>Single-pass type III membrane protein.; Note: Present in lipid rafts.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2, using LAT (Phospho-Tyr255) Antibody.)

Western Blot (WB)

(Western blot analysis of LAT (Phospho-Tyr255) using H2O2 treated 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

Testing Data

(At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Related Product Information for anti-LAT antibody

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

NCBI and Uniprot Product Information

• NCBI GI #: 62739155
• NCBI GeneID: 27040
• NCBI Accession #: NP_001014987.1
• NCBI GenBank Nucleotide #: NM_001014987.1
• UniProt Accession #: O43561; O43919
• Molecular Weight: 27,930 Da
• NCBI Official Full Name: linker for activation of T-cells family member 1 isoform b
• NCBI Official Synonym Full Names: linker for activation of T cells
• NCBI Official Symbol: LAT
• NCBI Official Synonym Symbols: LAT1; pp36
• NCBI Protein Information: linker for activation of T-cells family member 1; p36-38; 36 kDa phospho-tyrosine adapter protein; 36 kDa phospho-tyrosine adaptor protein; linker for activation of T cells, transmembrane adaptor
• UniProt Protein Name: Linker for activation of T-cells family member 1
• Protein Family: Latexin
• UniProt Gene Name: LAT
• UniProt Synonym Gene Names: pp36
• UniProt Entry Name: LAT_HUMAN

NCBI Description

The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules. Ref.7

Subunit structure: When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB, SKAP2 and CLNK

By similarity. Interacts with FCGR1A. Interacts with GRB2, PLCG1 and THEMIS upon TCR activation in thymocytes

By similarity. Ref.1 Ref.6 Ref.7 Ref.8 Ref.9 Ref.10 Ref.11

Subcellular location: Cell membrane; Single-pass type III membrane protein. Note: Present in lipid rafts. Ref.1 Ref.5

Tissue specificity: Expressed in thymus, T-cells, NK cells, mast cells and, at lower levels, in spleen. Present in T-cells but not B-cells (at protein level). Ref.1 Ref.17

Post-translational modification: Phosphorylated on tyrosines by ZAP70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ. Phosphorylated by ITK leading to the recruitment of VAV1 to LAT-containing complexes. Ref.1 Ref.10 Ref.12 Ref.13 Ref.14Palmitoylation of Cys-26 and Cys-29 is required for raft targeting and efficient phosphorylation. Ref.5

Miscellaneous: Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell-induced activation of PLC, maybe by inducing the dephosphorylation of LAT.