LAT recombinant antibody

Phospho-LAT (Y191) Antibody

Cat. Num. AAA7114855

• Gene Names: LAT; LAT1; pp36; IMD52
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation
• Purity: Affinity-Chromatography
• Synonyms: LAT; Monoclonal Recombinant Antibody; Phospho-LAT (Y191) Antibody; Linker for activation of T-cells family member 1; 36 kDa phospho-tyrosine adapter protein; pp36; p36-38; LAT recombinant antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Purity/Purification: Affinity-Chromatography
• Form/Format: Liquid; In phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Sequence Length: 233

• Applicable Applications for LAT recombinant antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:5000; IHC: 1:50-1:200; IF: 1:20-1:200; IP: 1:200-1:1000
• Immunogen Species: Human
• Immunogen: A synthesized peptide derived from human Phospho-LAT (Y191)
• Conjugate: Non-Conjugated
• Preparation and Storage: Upon receipt, store at-20 degree C or-80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western BlotPositive WB detected in?Jurkat whole cell lysate,Hela whole cell lysate,HepG2 whole cell lysate(treated with EGF or Pervanadate)All lanes?Phospho-LAT antibody at 2.9ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 38 KDaObserved band size: 38 KDa)

Immunohistochemistry (IHC)

(IHC image of CSB-RA012767A191phHU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunohistochemistry (IHC)

(IHC image of CSB-RA012767A191phHU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)

Immunofluorescence (IF)

(Immunofluorescence staining of Hela cells with CSB-RA012767A191phHU at 1:100,counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).)

Immunoprecipitation (IP)

(Immunoprecipitating Phospho-LAT in A549 whole cell lysateLane 1: Rabbit control IgG(1ug)instead of CSB-RA012767A191phHU in A549 whole cell lysate.For western blotting,a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)Lane 2: CSB-RA012767A191phHU(3ug)+ A549 whole cell lysate(1mg)Lane 3: A549 whole cell lysate (20ug))

Related Product Information for LAT recombinant antibody

Required for TCR (T-cell antigen receptor)-and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.

Product Categories/Family for LAT recombinant antibody

Recombinant Antibody; Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 62739155
• NCBI GeneID: 27040
• NCBI Accession #: NP_001014987.1
• NCBI GenBank Nucleotide #: NM_001014987.1
• UniProt Accession #: O43561; O43919; B7WPI0; C7C5T6; G5E9K3
• Molecular Weight: 24,985 Da
• NCBI Official Full Name: linker for activation of T-cells family member 1 isoform b
• NCBI Official Synonym Full Names: linker for activation of T cells
• NCBI Official Symbol: LAT
• NCBI Official Synonym Symbols: LAT1; pp36; IMD52
• NCBI Protein Information: linker for activation of T-cells family member 1
• UniProt Protein Name: Linker for activation of T-cells family member 1
• Protein Family: Latexin
• UniProt Gene Name: LAT
• UniProt Synonym Gene Names: pp36

NCBI Description

The protein encoded by this gene is phosphorylated by ZAP-70/Syk protein tyrosine kinases following activation of the T-cell antigen receptor (TCR) signal transduction pathway. This transmembrane protein localizes to lipid rafts and acts as a docking site for SH2 domain-containing proteins. Upon phosphorylation, this protein recruits multiple adaptor proteins and downstream signaling molecules into multimolecular signaling complexes located near the site of TCR engagement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules.